Serum steroid profile captures metabolic phenotypes in adults with classic congenital adrenal hyperplasia

Chang Ho Ahn, Jaeyoon Shim, Han Na Jang, Young Ah Lee, Sang Won Lee, Man Ho Choi, Jung Hee Kim

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Adult patients with classic congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency have an increased risk of metabolic diseases. We aimed to investigate whether liquid chromatography-mass spectrometry (LC-MS)-based serum steroid profiling reveals metabolic phenotypes in adults with classic CAH. Design and methods: This study prospectively enrolled 63 adult patients with CAH and 38 healthy volunteers. The levels of the 24 steroids were quantified in the morning serum using LC-MS. Unsupervised clustering algorithms were applied to the serum steroid profiles to identify unique patterns associated with metabolic syndrome. Results: Serum steroid profiles of patients with CAH were clearly delineated from those of healthy controls with a higher degree of interindividual heterogeneity. The unsupervised clustering algorithm divided CAH patients into two clusters based on serum steroid profile. Cluster 2 showed higher serum levels of glucocorticoids and androgens than cluster 1. The prevalence of metabolic syndrome was significantly higher in cluster 2 than in cluster 1 (37.8 % vs. 5.6 %, P = 0.011). Other clinical characteristics, including age, sex, body mass index, CAH subtypes, and glucocorticoid dose, did not differ between the two clusters. The multivariate logistic regression model of selective 15 steroids could discriminate metabolic syndrome in patients with CAH with an area under the receiver operating characteristic curve of 0.832 (95 % confidence interval:0.732–0.933). Conclusions: Serum steroid profiles can be valuable biomarkers for estimating metabolic risk in adult patients with CAH.

Original languageEnglish
Article number106374
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume234
DOIs
Publication statusPublished - 2023 Nov

Bibliographical note

Publisher Copyright:
© 2023 Elsevier Ltd

Keywords

  • 21-hydroxylase deficiency
  • Congenital adrenal hyperplasia
  • Mass spectrometry
  • Metabolic syndrome
  • Steroid profiling

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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