TY - JOUR
T1 - Sideroxylin (Callistemon lanceolatus) suppressed cell proliferation and increased apoptosis in ovarian cancer cells accompanied by mitochondrial dysfunction, the generation of reactive oxygen species, and an increase of lipid peroxidation
AU - Park, Sunwoo
AU - Lim, Whasun
AU - Jeong, Wonsik
AU - Bazer, Fuller W.
AU - Lee, Dongho
AU - Song, Gwonhwa
N1 - Funding Information:
Korea Health Technology R&D Project; Korea Health Industry Development Institute funded by the Ministry of Health & Welfare, Republic of Korea, Grant numbers: HI15C0810, HI17C0929
Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2018/11
Y1 - 2018/11
N2 - Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.
AB - Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells). Additionally, sideroxylin activated the phosphorylation of ERK1/2, JNK, P38, and MAPK proteins and the use of LY294002, U0126, SB203580, and SP600125 to block their phosphorylation, respectively, in ES2 and OV90 cells. Collectively, the results of present study indicated that sideroxylin was a novel therapeutic agent to combat the proliferation of ovarian cancer cells through the induction of mitochondrial dysfunction and the activation of PI3 K and MAPK signal transduction.
KW - Callistemon lanceolatus
KW - apoptosis
KW - ovarian cancer
KW - sideroxylin
KW - signal transduction
UR - http://www.scopus.com/inward/record.url?scp=85054075702&partnerID=8YFLogxK
U2 - 10.1002/jcp.26540
DO - 10.1002/jcp.26540
M3 - Article
C2 - 29904922
AN - SCOPUS:85054075702
SN - 0021-9541
VL - 233
SP - 8597
EP - 8604
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 11
ER -
