Silver nanoparticles induce Egr-1-dependent psoriasin expression via the ERK and p38 pathways

C. M. Kim, S. H. Jeong, H. Lee, H. J. Ryu, S. W. Son

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Background: Silver nanoparticles (Ag-NPs) can prevent bacterial infection and improve cutaneous wound healing owing to their antimicrobial activity. However, the mechanism of their antimicrobial activity is poorly understood. Aim: To determine the mechanistic relationship between Ag-NP treatment and expression of psoriasin. Methods: Human epidermal keratinocytes, neonatal (HEKn) were used. Psoriasin mRNA expression was measured by reverse transcription PCR and real-time PCR. Western blotting was performed to verify expression of early growth response-1 (Egr-1) and psoriasin, and phosphorylation of mitogen-activated protein kinase (MAPK). Psoriasin promoter activity by Egr-1 was detected by a luciferase assay. Results: Treatment of HEKn with Ag-NPs induced psoriasin mRNA and protein expression. Upregulation of psoriasin promoter activity was also observed in the luciferase assay. Ag-NPs increased Egr-1 expression, promoter activity and nuclear translocation in HEKn. Psoriasin luciferase activity was increased in HEKn transfected with Egr-1 pcDNA 3.1. Ag-NPs activated MAPK pathways including the extracellular signal-regulated kinase (ERK), p38, and c-Jun-N-terminal kinase (JNK) pathways. The upregulation of Egr-1 expression by Ag-NP stimulation was inhibited by ERK and p38 inhibitors, but not by a JNK inhibitor. Psoriasin expression was reduced in Egr-1 small interfering RNA-transfected HEKn. Conclusions: Ag-NP treatment induces upregulation of psoriasin expression through Egr-1 expression. We suggest that the ERK and p38 pathways are involved in Egr-1-dependent psoriasin expression.

Original languageEnglish
Pages (from-to)390-396
Number of pages7
JournalClinical and Experimental Dermatology
Issue number4
Publication statusPublished - 2019 Jun

Bibliographical note

Funding Information:
This work was supported by the National Research Foundation of Korea Grant, funded by the Korean Government (NRF-2016R1C1B1014180).

Publisher Copyright:
© 2018 British Association of Dermatologists

ASJC Scopus subject areas

  • Dermatology


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