Simple and sensitive method of microcantilever-based DNA detection using nanoparticles conjugates

  • Byung Hak Cha
  • , Sang Myung Lee
  • , Kyo Seon Hwang
  • , Sang Kyung Kim
  • , Yoon Sik Lee
  • , Byeong Kwon Ju
  • , Tae Song Kim

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    3 Citations (Scopus)

    Abstract

    As the real sample diagnosis using resonance-based microcantilevers, a simple method is strongly suggested that capture probe immobilized on the cantilever directly hybridize with nanoparticles conjugated detection probe. Two different dimensions of microfabricated piezoelectric cantilevers and two different sizes of nanoparticles were used in this experiment. As a result, the most suitable conditions, the 30 × 90 μm2 (width × length) of the cantilever and the 140nm of the nanoparticles, were chosen for this method. And quantitative analysis was carried out in different concentrations of nanoparticles conjugated detection probe which are 1 μg/mL, 10 μg/mL and 100 μg/mL. In addition, the sensitivity of this method was demonstrated through the SNP (single nucleotide polymorphism) detection by comparing with quantitative data of hybridization in full matched sequence at same concentration.

    Original languageEnglish
    Title of host publication3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
    Pages160-163
    Number of pages4
    DOIs
    Publication statusPublished - 2008
    Event3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008 - Sanya, China
    Duration: 2008 Jan 62008 Jan 9

    Publication series

    Name3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS

    Other

    Other3rd IEEE International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2008
    Country/TerritoryChina
    CitySanya
    Period08/1/608/1/9

    Keywords

    • Mass enhancement and SNP
    • Microcantilever

    ASJC Scopus subject areas

    • Control and Systems Engineering
    • Electrical and Electronic Engineering

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