Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim; Sang Keun Woo; Kwang Il Kim; Tae Sup Lee; Chan Wha Kim; Joo Hyun Kang; Byung Il Kim; Sang Moo Lim; Kyo Chul Lee; Yong Jin Lee

doi:10.1021/acsmedchemlett.5b00021

Simple methods for tracking stem cells with ⁶⁴Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim, Sang Keun Woo, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Joo Hyun Kang, Byung Il Kim, Sang Moo Lim, Kyo Chul Lee, Yong Jin Lee

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The purpose of this study was to evaluate ⁶⁴Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (⁶⁴Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with ⁶⁴Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

Original language	English
Pages (from-to)	528-530
Number of pages	3
Journal	ACS Medicinal Chemistry Letters
Volume	6
Issue number	5
DOIs	https://doi.org/10.1021/acsmedchemlett.5b00021
Publication status	Published - 2015 May 14

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

Keywords

Adipose-derived stem cells
PET
copper-64
hexadecyl benzoate
radiotracer

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

Access to Document

10.1021/acsmedchemlett.5b00021

Cite this

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title = "Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate",

abstract = "The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.",

keywords = "Adipose-derived stem cells, PET, copper-64, hexadecyl benzoate, radiotracer",

author = "Kim, {Min Hwan} and Woo, {Sang Keun} and Kim, {Kwang Il} and Lee, {Tae Sup} and Kim, {Chan Wha} and Kang, {Joo Hyun} and Kim, {Byung Il} and Lim, {Sang Moo} and Lee, {Kyo Chul} and Lee, {Yong Jin}",

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AU - Kim, Min Hwan

AU - Woo, Sang Keun

AU - Kim, Kwang Il

AU - Lee, Tae Sup

AU - Kim, Chan Wha

AU - Kang, Joo Hyun

AU - Kim, Byung Il

AU - Lim, Sang Moo

AU - Lee, Kyo Chul

AU - Lee, Yong Jin

PY - 2015/5/14

Y1 - 2015/5/14

N2 - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

AB - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

KW - Adipose-derived stem cells

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KW - copper-64

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