Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim, Sang Keun Woo, Kwang Il Kim, Tae Sup Lee, Chan Wha Kim, Joo Hyun Kang, Byung Il Kim, Sang Moo Lim, Kyo Chul Lee, Yong Jin Lee

    Research output: Contribution to journalArticlepeer-review

    15 Citations (Scopus)

    Abstract

    The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

    Original languageEnglish
    Pages (from-to)528-530
    Number of pages3
    JournalACS Medicinal Chemistry Letters
    Volume6
    Issue number5
    DOIs
    Publication statusPublished - 2015 May 14

    Bibliographical note

    Publisher Copyright:
    © 2015 American Chemical Society.

    Keywords

    • Adipose-derived stem cells
    • PET
    • copper-64
    • hexadecyl benzoate
    • radiotracer

    ASJC Scopus subject areas

    • Biochemistry
    • Drug Discovery
    • Organic Chemistry

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