Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim; Sang Keun Woo; Kwang Il Kim; Tae Sup Lee; Chan Wha Kim; Joo Hyun Kang; Byung Il Kim; Sang Moo Lim; Kyo Chul Lee; Yong Jin Lee

doi:10.1021/acsmedchemlett.5b00021

Simple methods for tracking stem cells with ⁶⁴Cu-labeled DOTA-hexadecyl-benzoate

Min Hwan Kim
, Sang Keun Woo
, Kwang Il Kim
, Tae Sup Lee
, Chan Wha Kim
, Joo Hyun Kang
, Byung Il Kim
, Sang Moo Lim
, Kyo Chul Lee^*
, Yong Jin Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The purpose of this study was to evaluate ⁶⁴Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (⁶⁴Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with ⁶⁴Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

Original language	English
Pages (from-to)	528-530
Number of pages	3
Journal	ACS Medicinal Chemistry Letters
Volume	6
Issue number	5
DOIs	https://doi.org/10.1021/acsmedchemlett.5b00021
Publication status	Published - 2015 May 14

Bibliographical note

Publisher Copyright:
© 2015 American Chemical Society.

Keywords

Adipose-derived stem cells
PET
copper-64
hexadecyl benzoate
radiotracer

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

Access to Document

10.1021/acsmedchemlett.5b00021

Cite this

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title = "Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate",

abstract = "The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99\%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50\%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.",

keywords = "Adipose-derived stem cells, PET, copper-64, hexadecyl benzoate, radiotracer",

author = "Kim, \{Min Hwan\} and Woo, \{Sang Keun\} and Kim, \{Kwang Il\} and Lee, \{Tae Sup\} and Kim, \{Chan Wha\} and Kang, \{Joo Hyun\} and Kim, \{Byung Il\} and Lim, \{Sang Moo\} and Lee, \{Kyo Chul\} and Lee, \{Yong Jin\}",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

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T1 - Simple methods for tracking stem cells with 64Cu-labeled DOTA-hexadecyl-benzoate

AU - Kim, Min Hwan

AU - Woo, Sang Keun

AU - Kim, Kwang Il

AU - Lee, Tae Sup

AU - Kim, Chan Wha

AU - Kang, Joo Hyun

AU - Kim, Byung Il

AU - Lim, Sang Moo

AU - Lee, Kyo Chul

AU - Lee, Yong Jin

PY - 2015/5/14

Y1 - 2015/5/14

N2 - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

AB - The purpose of this study was to evaluate 64Cu-labeled hexadecyl-1,4,7,10-tetraazacyclododecane-tetraacetic acid-benzoate (64Cu-DOTA-HB) (1) as positron emission tomography (PET) radiotracer for stem cell imaging. Hexadecyl-DOTA-benzoate (DOTA-HB) (2) was efficiently labeled with 64Cu (>99%), and cell labeling efficiency with adipose-derived stem cells (ADSCs) was over 50%. Labeling with 1 did not compromise cell viability. In the PET imaging, intramuscularly transplanted 1-labeled ADSCs were monitored for 18 h in normal rat heart. These results indicate that 1 can be utilized as a promising radiotracer for monitoring of transplanted stem cells.

KW - Adipose-derived stem cells

KW - PET

KW - copper-64

KW - hexadecyl benzoate

KW - radiotracer

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