Simultaneous imaging of mitochondria and lysosomes by using two-photon fluorescent probes

Ji Hee Han; Sun Keun Park; Chang Su Lim; Min Kyu Park; Hyung Joong Kim; Hwan Myung Kim; Bong Rae Cho

doi:10.1002/chem.201203452

Simultaneous imaging of mitochondria and lysosomes by using two-photon fluorescent probes

Ji Hee Han, Sun Keun Park, Chang Su Lim, Min Kyu Park, Hyung Joong Kim, Hwan Myung Kim, Bong Rae Cho

Research output: Contribution to journal › Article › peer-review

44 Citations (Scopus)

Abstract

Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).

Original language	English
Pages (from-to)	15246-15249
Number of pages	4
Journal	Chemistry - A European Journal
Volume	18
Issue number	48
DOIs	https://doi.org/10.1002/chem.201203452
Publication status	Published - 2012 Nov 26

Keywords

fluorescence
fluorescent probes
imaging agents
lysosomes
mitochondria

ASJC Scopus subject areas

Catalysis
General Chemistry
Organic Chemistry

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10.1002/chem.201203452

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abstract = "Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).",

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AU - Han, Ji Hee

AU - Park, Sun Keun

AU - Lim, Chang Su

AU - Park, Min Kyu

AU - Kim, Hyung Joong

AU - Kim, Hwan Myung

AU - Cho, Bong Rae

PY - 2012/11/26

Y1 - 2012/11/26

N2 - Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).

AB - Dual-color imaging: Two-photon probes for lysosomes (BLT-blue) and mitochondria (FMT-green) that can be excited by 750 nm femtosecond laser pulses and emit at λ=400-450 and 550-600 nm, respectively, are reported. They allow simultaneous dual-color imaging of lysosomes and mitochondria in live cells and tissues for a long period of time with minimum interference from pH, photobleaching, or cytotoxicity (see scheme).

KW - fluorescence

KW - fluorescent probes

KW - imaging agents

KW - lysosomes

KW - mitochondria

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Simultaneous imaging of mitochondria and lysosomes by using two-photon fluorescent probes

Abstract

Keywords

ASJC Scopus subject areas

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