Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans

Nicholas F. Trojanowski; Christopher Fang-Yen

doi:10.1007/978-1-4939-2842-2_9

Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans

Nicholas F. Trojanowski, Christopher Fang-Yen

Research output: Chapter in Book/Report/Conference proceeding › Chapter

8 Citations (Scopus)

Abstract

Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	105-119
Number of pages	15
DOIs	https://doi.org/10.1007/978-1-4939-2842-2_9
Publication status	Published - 2015

Publication series

Name	Methods in Molecular Biology
Volume	1327
ISSN (Print)	1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

C. elegans
Feeding behavior
In vivo optogenetics
Optogenetics
Pharynx
Small circuits

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1007/978-1-4939-2842-2_9

Cite this

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AU - Fang-Yen, Christopher

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PY - 2015

Y1 - 2015

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AB - Optogenetic approaches have proven powerful for examining the role of neural circuits in generating behaviors, especially in systems where electrophysiological manipulation is not possible. Here we describe a method for optogenetically manipulating single pharyngeal neurons in intact C. elegans while monitoring pharyngeal behavior. This approach provides bidirectional and dynamic control of pharyngeal neural activity simultaneously with a behavioral readout and has allowed us to test hypotheses about the roles of individual pharyngeal neurons in regulating feeding behavior.

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KW - Feeding behavior

KW - In vivo optogenetics

KW - Optogenetics

KW - Pharynx

KW - Small circuits

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T3 - Methods in Molecular Biology

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EP - 119

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Simultaneous optogenetic stimulation of individual pharyngeal neurons and monitoring of feeding behavior in intact C. elegans

Abstract

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Bibliographical note

Keywords

ASJC Scopus subject areas

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