Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

Saurabh Sudha Dhiman; Jung Rim Haw; Dayanand Kalyani; Vipin C. Kalia; Yun Chan Kang; Jung Kul Lee

doi:10.1016/j.biortech.2014.11.059

Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

Saurabh Sudha Dhiman, Jung Rim Haw, Dayanand Kalyani, Vipin C. Kalia, Yun Chan Kang, Jung Kul Lee

Department of Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.

Original language	English
Pages (from-to)	50-57
Number of pages	8
Journal	Bioresource technology
Volume	179
DOIs	https://doi.org/10.1016/j.biortech.2014.11.059
Publication status	Published - 2015 Mar 1

Keywords

Ethanol
Fungal consortia
Pretreatment
Reducing sugars
Saccharification

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.biortech.2014.11.059

Cite this

@article{c9d336a2fb94486aaa165a0aff64ca79,

title = "Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium",

abstract = "Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.",

keywords = "Ethanol, Fungal consortia, Pretreatment, Reducing sugars, Saccharification",

author = "Dhiman, {Saurabh Sudha} and Haw, {Jung Rim} and Dayanand Kalyani and Kalia, {Vipin C.} and Kang, {Yun Chan} and Lee, {Jung Kul}",

note = "Funding Information: This research was supported a Grant from the Intelligent Synthetic Biology Center of Global Frontier Project (2011-0031955) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea . This work was supported by 2013 KU Brain Pool fellowship of Konkuk University . Funding Information: This work was supported by the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea ( 201320200000420 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd.",

year = "2015",

month = mar,

day = "1",

doi = "10.1016/j.biortech.2014.11.059",

language = "English",

volume = "179",

pages = "50--57",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Simultaneous pretreatment and saccharification

T2 - Green technology for enhanced sugar yields from biomass using a fungal consortium

AU - Dhiman, Saurabh Sudha

AU - Haw, Jung Rim

AU - Kalyani, Dayanand

AU - Kalia, Vipin C.

AU - Kang, Yun Chan

AU - Lee, Jung Kul

N1 - Funding Information: This research was supported a Grant from the Intelligent Synthetic Biology Center of Global Frontier Project (2011-0031955) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea . This work was supported by 2013 KU Brain Pool fellowship of Konkuk University . Funding Information: This work was supported by the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP), granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea ( 201320200000420 ). Publisher Copyright: © 2014 Elsevier Ltd.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.

AB - Two different biomasses were subjected to simultaneous pretreatment and saccharification (SPS) using a cocktail of hydrolytic and oxidizing enzymes. Application of a novel laccase as a detoxifying agent caused the removal of 49.8% and 32.6% of phenolic contents from the soaked rice straw and willow, respectively. Hydrolysis of soaked substrates using a newly developed fungal consortium resulted in saccharification yield of up to 74.2% and 63.6% for rice straw and willow, respectively. A high saccharification yield was obtained with soaked rice straw and willow without using any hazardous chemicals. The efficiency of each step related to SPS was confirmed by atomic force microscopy. The suitability of the developed SPS process was further confirmed by converting the hydrolysate from the process into bioethanol with 72.4% sugar conversion efficiency. To the best of our knowledge, this is the first report on the development of a less tedious, single-pot, and eco-friendly SPS methodology.

KW - Ethanol

KW - Fungal consortia

KW - Pretreatment

KW - Reducing sugars

KW - Saccharification

UR - http://www.scopus.com/inward/record.url?scp=84916918794&partnerID=8YFLogxK

U2 - 10.1016/j.biortech.2014.11.059

DO - 10.1016/j.biortech.2014.11.059

M3 - Article

C2 - 25514402

AN - SCOPUS:84916918794

SN - 0960-8524

VL - 179

SP - 50

EP - 57

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Simultaneous pretreatment and saccharification: Green technology for enhanced sugar yields from biomass using a fungal consortium

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this