Single-and repeated-dose oral toxicity in rats and bacterial reverse mutation test of Morus alba L. Extracts

Taewon Han, Min Young Um, Young Hee Lim, Jeong Keun Kim, In Ho Kim

Research output: Contribution to journalArticlepeer-review


This study was carried out to evaluate the toxicity of ethanolic extracts of Morus alba L. branch (ME). In the reverse mutation test, Salmonella Typhimurium TA98, TA100, TA1535, TA1357, and Escherichia coli WP2uvrA were used to estimate the mutagenic potential of ME. Sprague-Dawley rats were orally administered ME at levels of 1,250, 2,500, and 5,000 mg/kg for the single-dose toxicity test and 500, 1,000, and 2,000 mg/kg/d for the repeated-dose toxicity test for 28 consecutive days. As expected, reverse mutation was not detected at any concentration of ME, regardless of application of the metabolic activation system with or without S9 mix. In the single-dose toxicity test, ME caused neither significant visible signs of toxicity nor mortality in rats, and LD50 was estimated to be over 5,000 mg/kg. In the repeated-dose toxicity test, ME administration at 500, 1,000, and 2,000 mg/kg for 28 days to male or female rats did not result in mortality. Similarly, no toxicologically significant treatment-related changes in body weight, food intake, or organ weights were noted. Several hematological and biochemical parameters in both genders showed significant differences, but these were within normal ranges. These results support the safe use of ME.

Original languageEnglish
Pages (from-to)1406-1413
Number of pages8
JournalJournal of the Korean Society of Food Science and Nutrition
Issue number10
Publication statusPublished - 2016 Oct 1


  • Morus alba ethanol extracts
  • Mutagenic toxicity
  • Repeated-dose toxicity
  • Single-dose toxicity

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics


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