Single enzyme nanoparticles

Jungbae Kim, Jay W. Grate

Research output: Contribution to conferencePaperpeer-review

1 Citation (Scopus)


Nanoparticles and nanoparticle-based materials are attracting great interest for their unique properties and potential for application in diverse areas. We have developed a synthetic approach for the preparation of single enzyme nanoparticles (SENs), in which each enzyme molecule is surrounded by a hybrid organic/inorganic polymer network. The synthetic procedure consists of modification and two orthogonal polymerization steps. This SEN approach has been successful in stabilizing several enzymes in a vivid way. In addition, the nanoscale structure around the enzyme is sufficiently thin enough not to impose a significant mass transfer limitation on the substrate. Because these nanoparticles remain soluble or suspended in solutions, they can be processed into a variety of forms. They can be used in solution, cast into thin films, or adsorbed within larger mesoporoous structures. Given enzymatic specificity and the great diversity reactions catalyzed by enzymes, coupled with the flexibility in the use of single enzyme nanoparticles, these new nanostructures are promising for many applications including biosensors and bioconversion.

Original languageEnglish
Number of pages1
Publication statusPublished - 2005
Event05AIChE: 2005 AIChE Annual Meeting and Fall Showcase - Cincinnati, OH, United States
Duration: 2005 Oct 302005 Nov 4


Other05AIChE: 2005 AIChE Annual Meeting and Fall Showcase
Country/TerritoryUnited States
CityCincinnati, OH

ASJC Scopus subject areas

  • Engineering(all)


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