Single enzyme nanoparticles armored by a thin silicate network: Single enzyme caged nanoparticles

Sung Gil Hong, Byoung Chan Kim, Hyon Bin Na, Jinwoo Lee, Jongkyu Youn, Seung Wook Chung, Chang Won Lee, Byoungsoo Lee, Han Sol Kim, Erik Hsiao, Seong H. Kim, Byung Gee Kim, Hyun Gyu Park, Ho Nam Chang, Taeghwan Hyeon, Jonathan S. Dordick, Jay W. Grate, Jungbae Kim

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)


For the encapsulation of biomolecules in inorganic materials, we have developed a unique enzyme-silicate conjugate material that consists of a self-assembled molecularly thin silicate layer on the surface of each individual enzyme molecule. The enzyme-silicate conjugate materials, called single enzyme caged nanoparticles (SECNs), were synthesized via the silica polymerization on the surface of enzyme molecule after solubilizing each enzyme molecule in hexane by using a tiny amount of surfactant, called “ion-pairing”. SECNs possess near native enzyme activity in aqueous media with minimal substrate diffusional limitations, and are highly stable under the protection of silicate network cage. Due to their nearly molecular size, SECNs can also be adsorbed into mesoporous silica materials to yield robust and easily-recyclable enzymatic systems that can be used in a number of potential biocatalytic applications such as diagnostics, biosensors, biotransformations, biofuel production, bioremediation and CO2 capture.

Original languageEnglish
Pages (from-to)510-515
Number of pages6
JournalChemical Engineering Journal
Publication statusPublished - 2017

Bibliographical note

Funding Information:
This research was supported by Global Research Laboratory Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT & Future Planning (2014K1A1A2043032).

Publisher Copyright:
© 2017 Elsevier B.V.


  • Enzyme catalysis
  • Enzyme immobilization
  • Enzyme stabilization
  • Enzymes
  • Single enzyme caged nanoparticles

ASJC Scopus subject areas

  • General Chemistry
  • Environmental Chemistry
  • General Chemical Engineering
  • Industrial and Manufacturing Engineering


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