Abstract
Electrospun polymer nanofibers with entrapped magnetic nanoparticles (magnetic NP-NF) represent a novel scaffold substrate that can be functionalized for single-step isolation and activation of specific lymphocyte subsets. Using a surface-embedded T cell receptor ligand/trigger (anti-CD3 monoclonal antibody), we demonstrate, as proof of principle, the use of magnetic NP-NF to specifically isolate, enrich, and activate CD3 + T cells from a heterogeneous cell mixture, leading to preferential expansion of CD8 +CD3 + T cells. The large surface area, adjustable antibody density, and embedded paramagnetic properties of the NP-NF permitted enhanced activation and expansion; its use represents a strategy that is amenable to an efficient selection process for adoptive cellular therapy as well as for the isolation of other cellular subsets for downstream translational applications.
Original language | English |
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Pages (from-to) | 4018-4024 |
Number of pages | 7 |
Journal | Nano Letters |
Volume | 12 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2012 Aug 8 |
Keywords
- CD4 T lymphocytes
- CD8 T lymphocytes
- Electrospun magnetic nanofiber
- anti-CD3 monoclonal antibody
- immune cell therapy
ASJC Scopus subject areas
- Bioengineering
- General Chemistry
- General Materials Science
- Condensed Matter Physics
- Mechanical Engineering