Site-specific incorporation of μ-N-crotonyllysine into histones

Chan Hyuk Kim; Mingchao Kang; Hak Joong Kim; Abhishek Chatterjee; Peter G. Schultz

doi:10.1002/anie.201203349

Site-specific incorporation of μ-N-crotonyllysine into histones

Chan Hyuk Kim, Mingchao Kang, Hak Joong Kim, Abhishek Chatterjee, Peter G. Schultz

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

89 Citations (Scopus)

Abstract

A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.

Original language	English
Pages (from-to)	7246-7249
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	51
Issue number	29
DOIs	https://doi.org/10.1002/anie.201203349
Publication status	Published - 2012 Jul 16

Keywords

Crotonylations
Histones
Nonnatural amino acids
Post-translational modifications

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.201203349

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abstract = "A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.",

keywords = "Crotonylations, Histones, Nonnatural amino acids, Post-translational modifications",

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AU - Kang, Mingchao

AU - Kim, Hak Joong

AU - Chatterjee, Abhishek

AU - Schultz, Peter G.

PY - 2012/7/16

Y1 - 2012/7/16

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AB - A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.

KW - Crotonylations

KW - Histones

KW - Nonnatural amino acids

KW - Post-translational modifications

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U2 - 10.1002/anie.201203349

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IS - 29

ER -

Site-specific incorporation of μ-N-crotonyllysine into histones

Abstract

Keywords

ASJC Scopus subject areas

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