A novel post-translationally modified amino acid, crotonyllysine (Kcr), was genetically incorporated into proteins in bacterial and mammalian cells using an evolved pyrrolysyl-tRNA/synthetase-tRNA pair. The ability to produce histones with homogenous, site-specific Kcr modifications will be valuable in elucidating the biological role of this recently identified post-translational modification.
|Number of pages||4|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 2012 Jul 16|
- Nonnatural amino acids
- Post-translational modifications
ASJC Scopus subject areas