Size-controllable quartz nanostructure for signal enhancement of DNA chip

Jung Suk Kim; Jae Bum Cho; Bo Gi Park; Wonbae Lee; Kyu Back Lee; Min Kyu Oh

doi:10.1016/j.bios.2010.09.010

Size-controllable quartz nanostructure for signal enhancement of DNA chip

Jung Suk Kim, Jae Bum Cho, Bo Gi Park, Wonbae Lee, Kyu Back Lee, Min Kyu Oh

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

A mask-free, cost-effective dry-etching method for the fabrication of height- and spacing-controlled, pillar-like nanostructures was established in order to detect DNA molecules. The height and spacing of the quartz nanostructure were regulated by successive O₂ and CF₄ reactive ion etching times. The height and spacing of the nanostructures were tuned between 118 and 269nm and between 107 and 161nm, respectively. Probe DNA was immobilized on the structure and hybridized with fluorescently-labeled target DNA. Increases in the height and spacing of the nanopillar structure positively correlated with the fluorescence intensity of bound DNA. Usage of the nanostructure increased the DNA detection limit by up to 100-fold.

Original language	English
Pages (from-to)	2085-2089
Number of pages	5
Journal	Biosensors and Bioelectronics
Volume	26
Issue number	5
DOIs	https://doi.org/10.1016/j.bios.2010.09.010
Publication status	Published - 2011 Jan 15

Keywords

DNA chip
DNA sensor
Nanopillar
Nanostructured quartz

ASJC Scopus subject areas

Biotechnology
Biophysics
Biomedical Engineering
Electrochemistry

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10.1016/j.bios.2010.09.010

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abstract = "A mask-free, cost-effective dry-etching method for the fabrication of height- and spacing-controlled, pillar-like nanostructures was established in order to detect DNA molecules. The height and spacing of the quartz nanostructure were regulated by successive O2 and CF4 reactive ion etching times. The height and spacing of the nanostructures were tuned between 118 and 269nm and between 107 and 161nm, respectively. Probe DNA was immobilized on the structure and hybridized with fluorescently-labeled target DNA. Increases in the height and spacing of the nanopillar structure positively correlated with the fluorescence intensity of bound DNA. Usage of the nanostructure increased the DNA detection limit by up to 100-fold.",

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AU - Kim, Jung Suk

AU - Cho, Jae Bum

AU - Park, Bo Gi

AU - Lee, Wonbae

AU - Lee, Kyu Back

AU - Oh, Min Kyu

PY - 2011/1/15

Y1 - 2011/1/15

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AB - A mask-free, cost-effective dry-etching method for the fabrication of height- and spacing-controlled, pillar-like nanostructures was established in order to detect DNA molecules. The height and spacing of the quartz nanostructure were regulated by successive O2 and CF4 reactive ion etching times. The height and spacing of the nanostructures were tuned between 118 and 269nm and between 107 and 161nm, respectively. Probe DNA was immobilized on the structure and hybridized with fluorescently-labeled target DNA. Increases in the height and spacing of the nanopillar structure positively correlated with the fluorescence intensity of bound DNA. Usage of the nanostructure increased the DNA detection limit by up to 100-fold.

KW - DNA chip

KW - DNA sensor

KW - Nanopillar

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Size-controllable quartz nanostructure for signal enhancement of DNA chip

Abstract

Keywords

ASJC Scopus subject areas

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