TY - JOUR
T1 - Size-dependent plasmonic responses of single gold nanoparticles for analysis of biorecognition
AU - Hwang, Woo Sung
AU - Truong, Phuoc Long
AU - Sim, Sang Jun
N1 - Funding Information:
This work was supported by the Korea Science and Engineering Foundation (KOSEF) National Research Laboratory (NRL) Program ( R0A-2008-000-20078-0 ), the Basic Science Research Program ( 2010-0027771 ), and a grant from the Carbon Dioxide Reduction & Sequestration Research Center ( DG2-201 ), one of the 21st Century Frontier Programs funded by the Ministry of Science and Technology of the Korean government of the Republic of Korea .
PY - 2012/2/1
Y1 - 2012/2/1
N2 - We report the use of plasmonic responses of single gold nanoparticles (AuNPs) with various sizes for the analysis of biomolecular recognition. We also describe the relationship between particle size and plasmonic response induced by the binding of receptors and target analytes. To investigate the plasmonic response of AuNPs, Rayleigh light scattering spectra were collected from individual AuNPs using a dark-field microspectroscopy system. Using prostate-specific antigen (PSA) as a model, the linear dynamic range was obtained in the concentration range of 10-4 to 10 ng/ml, with the smallest detectable concentration at 0.1 pg/ml corresponding to localized surface plasmon resonance (LSPR) λmax shifts of approximately 2.95 nm. This result indicates that individual AuNPs can be used for development of a very sensitive, robust, simple, and label-free biosensor to detect protein biomarkers. Furthermore, the method possesses great potential for monitoring other biological interactions.
AB - We report the use of plasmonic responses of single gold nanoparticles (AuNPs) with various sizes for the analysis of biomolecular recognition. We also describe the relationship between particle size and plasmonic response induced by the binding of receptors and target analytes. To investigate the plasmonic response of AuNPs, Rayleigh light scattering spectra were collected from individual AuNPs using a dark-field microspectroscopy system. Using prostate-specific antigen (PSA) as a model, the linear dynamic range was obtained in the concentration range of 10-4 to 10 ng/ml, with the smallest detectable concentration at 0.1 pg/ml corresponding to localized surface plasmon resonance (LSPR) λmax shifts of approximately 2.95 nm. This result indicates that individual AuNPs can be used for development of a very sensitive, robust, simple, and label-free biosensor to detect protein biomarkers. Furthermore, the method possesses great potential for monitoring other biological interactions.
KW - Biomolecular recognition
KW - Gold nanoparticle (AuNP)
KW - Localized surface plasmon resonance (LSPR)
KW - Resonant Rayleigh light scattering
UR - http://www.scopus.com/inward/record.url?scp=84855899216&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2011.11.001
DO - 10.1016/j.ab.2011.11.001
M3 - Article
C2 - 22146558
AN - SCOPUS:84855899216
SN - 0003-2697
VL - 421
SP - 213
EP - 218
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -