TY - JOUR
T1 - Size homeostasis in adherent cells studied by synthetic phase microscopy
AU - Sung, Yongjin
AU - Tzur, Amit
AU - Oh, Seungeun
AU - Choi, Wonshik
AU - Li, Victor
AU - Dasari, Ramachandra R.
AU - Yaqoob, Zahid
AU - Kirschner, Marc W.
PY - 2013/10/8
Y1 - 2013/10/8
N2 - The coupling of the rate of cell growth to the rate of cell division determines cell size, a defining characteristic that is central to cell function and, ultimately, to tissue architecture. The physiology of size homeostasis has fascinated generations of biologists, but the mechanism, challenged by experimental limitations, remains largely unknown. In this paper, we propose a unique optical method that can measure the dry mass of thick live cells as accurately as that for thin cells with high computational efficiency. With this technique, we quantify, with unprecedented accuracy, the asymmetry of division in lymphoblasts and epithelial cells. We can then use the Collins-Richmond model of conservation to compute the relationship between growth rate and cell mass. In attached epithelial cells, we find that due to the asymmetry in cell division and sizedependent growth rate, there is active regulation of cell size. Thus, like nonadherent cells, size homeostasis requires feedback control.
AB - The coupling of the rate of cell growth to the rate of cell division determines cell size, a defining characteristic that is central to cell function and, ultimately, to tissue architecture. The physiology of size homeostasis has fascinated generations of biologists, but the mechanism, challenged by experimental limitations, remains largely unknown. In this paper, we propose a unique optical method that can measure the dry mass of thick live cells as accurately as that for thin cells with high computational efficiency. With this technique, we quantify, with unprecedented accuracy, the asymmetry of division in lymphoblasts and epithelial cells. We can then use the Collins-Richmond model of conservation to compute the relationship between growth rate and cell mass. In attached epithelial cells, we find that due to the asymmetry in cell division and sizedependent growth rate, there is active regulation of cell size. Thus, like nonadherent cells, size homeostasis requires feedback control.
KW - Cell division asymmetry
KW - Cell dry mass
KW - Cell growth
KW - Interferometry
KW - Synthetic phase microscopy
UR - http://www.scopus.com/inward/record.url?scp=84885357849&partnerID=8YFLogxK
U2 - 10.1073/pnas.1315290110
DO - 10.1073/pnas.1315290110
M3 - Article
C2 - 24065823
AN - SCOPUS:84885357849
SN - 0027-8424
VL - 110
SP - 16687
EP - 16692
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 41
ER -