TY - JOUR
T1 - Sleep-promoting effects of the GABA/5-HTP mixture in vertebrate models
AU - Hong, Ki Bae
AU - Park, Yooheon
AU - Suh, Hyung Joo
N1 - Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT , and Future Planning ( 2014R1A2A1A11052187 ).
PY - 2016/9/1
Y1 - 2016/9/1
N2 - The aim of this study was to investigate the sleep-promoting effect of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) on sleep quality and quantity in vertebrate models. Pentobarbital-induced sleep test and electroencephalogram (EEG) analysis were applied to investigate sleep latency, duration, total sleeping time and sleep quality of two amino acids and GABA/5-HTP mixture. In addition, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. The GABA/5-HTP mixture significantly regulated the sleep latency, duration (p < 0.005), and also increased the sleep quality than single administration of the amino acids (p < 0.000). Long-term administration increased the transcript levels of GABAA receptor (1.37-fold, p < 0.000) and also increased the GABA content compared with the control group 12 h after administration (1.43-fold, p < 0.000). Our available evidence suggests that the GABA/5-HTP mixture modulates both GABAergic and serotonergic signaling. Moreover, the sleep architecture can be controlled by the regulation of GABAA receptor and GABA content with 5-HTP.
AB - The aim of this study was to investigate the sleep-promoting effect of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) on sleep quality and quantity in vertebrate models. Pentobarbital-induced sleep test and electroencephalogram (EEG) analysis were applied to investigate sleep latency, duration, total sleeping time and sleep quality of two amino acids and GABA/5-HTP mixture. In addition, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. The GABA/5-HTP mixture significantly regulated the sleep latency, duration (p < 0.005), and also increased the sleep quality than single administration of the amino acids (p < 0.000). Long-term administration increased the transcript levels of GABAA receptor (1.37-fold, p < 0.000) and also increased the GABA content compared with the control group 12 h after administration (1.43-fold, p < 0.000). Our available evidence suggests that the GABA/5-HTP mixture modulates both GABAergic and serotonergic signaling. Moreover, the sleep architecture can be controlled by the regulation of GABAA receptor and GABA content with 5-HTP.
KW - 5-Hydroxytryptophan
KW - Electroencephalogram
KW - Pentobarbital
KW - Sleep
KW - Vertebrate
KW - γ-Aminobutyric acid
UR - http://www.scopus.com/inward/record.url?scp=84966417117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84966417117&partnerID=8YFLogxK
U2 - 10.1016/j.bbr.2016.04.049
DO - 10.1016/j.bbr.2016.04.049
M3 - Article
C2 - 27150227
AN - SCOPUS:84966417117
SN - 0166-4328
VL - 310
SP - 36
EP - 41
JO - Behavioural Brain Research
JF - Behavioural Brain Research
ER -