Sleep-promoting effects of the GABA/5-HTP mixture in vertebrate models

Ki Bae Hong, Yooheon Park, Hyung Joo Suh

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


The aim of this study was to investigate the sleep-promoting effect of combined γ-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) on sleep quality and quantity in vertebrate models. Pentobarbital-induced sleep test and electroencephalogram (EEG) analysis were applied to investigate sleep latency, duration, total sleeping time and sleep quality of two amino acids and GABA/5-HTP mixture. In addition, real-time PCR and HPLC analysis were applied to analyze the signaling pathway. The GABA/5-HTP mixture significantly regulated the sleep latency, duration (p < 0.005), and also increased the sleep quality than single administration of the amino acids (p < 0.000). Long-term administration increased the transcript levels of GABAA receptor (1.37-fold, p < 0.000) and also increased the GABA content compared with the control group 12 h after administration (1.43-fold, p < 0.000). Our available evidence suggests that the GABA/5-HTP mixture modulates both GABAergic and serotonergic signaling. Moreover, the sleep architecture can be controlled by the regulation of GABAA receptor and GABA content with 5-HTP.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalBehavioural Brain Research
Publication statusPublished - 2016 Sept 1

Bibliographical note

Funding Information:
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT , and Future Planning ( 2014R1A2A1A11052187 ).


  • 5-Hydroxytryptophan
  • Electroencephalogram
  • Pentobarbital
  • Sleep
  • Vertebrate
  • γ-Aminobutyric acid

ASJC Scopus subject areas

  • Behavioral Neuroscience


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