Small RNA sequencing of small extracellular vesicles secreted by umbilical cord mesenchymal stem cells following replicative senescence

Chris Gunwoo Kim, Jae Kyung Lee, Geum-Joon Cho, Ok Sarah Shin, Jeong An Gim

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Background: Umbilical cord mesenchymal stem cells (UCMSC) are subsets of multipotent stem cells involved in immune modulation, tissue regeneration, and antimicrobial defense. Cellular senescence is associated with the onset of aging-related diseases and small extracellular vesicles (sEVs) are important mediators of senescence and aging. Objective: However, little is known about the role and function of microRNAs (miRNAs) carried by UCMSC-derived sEVs. To analyze the expression profiles of miRNAs secreted by senescent UCMSC, small RNA sequencing of the miRNAs within the sEVs was performed in this study. Methods: UCMSC cultures underwent serial passaging beyond passage number 20 to achieve replicative senescence, which was confirmed by various methods, including increased senescence-associated β-gal staining and cytokine secretion levels. sEVs derived from non-senescent and senescent UCMSC were isolated and characterized by nanoparticle tracking analysis, transmission electron microscopy, and immunoblot analysis. Results: Small RNA sequencing of the miRNAs within the sEVs revealed senescence-associated differences in the miRNA composition, as shown by the upregulation of miR-122-5p and miR-146a-5p, and downregulation of miR-125b-5p and miR-29-3p. In addition, total RNA sequencing analysis showed that PENK, ITGA8, and TSIX were upregulated, whereas AKR1B10, UNC13D, and IL21R were downregulated by replicative senescence in UCMSC. In sEVs, upregulated genes were linked to downregulated miRNAs, and vice versa. In the gene-concept network analysis, five gynecologic terms were retrieved. Conclusions: The study provides an insight into the cellular characteristics of UCMSC following replicative senescence and emphasizes the importance of monitoring passage numbers of UCMSC for further therapeutic use.

Original languageEnglish
Pages (from-to)347-358
Number of pages12
JournalGenes and Genomics
Volume45
Issue number3
DOIs
Publication statusPublished - 2023 Mar
Externally publishedYes

Bibliographical note

Funding Information:
This research was funded by MD-PhD/Medical Scientist Training Program through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea, and the Basic Science Research Program of the National Research Foundation of Korea (NRF) by the Ministry of Science, ICT & Future Planning (NRF-2019R1A2C1005961). This research was also funded by the Basic Science Research Program through NRF by the Ministry of Education (NRF-2020R1I1A1A01052701).

Publisher Copyright:
© 2022, The Author(s) under exclusive licence to The Genetics Society of Korea.

Keywords

  • Senescence
  • Small extracellular vesicles
  • UCMSC
  • microRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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