Smoking status-dependent association of the 27-bp repeat polymorphism in intron 4 of endothelial nitric oxide synthase gene with plasma nitric oxide concentrations

Suin Yoon, Jesung Moon, Chol Shin, Eunkyung Kim, Sangmee Ahn Jo, Inho Jo

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31 Citations (Scopus)


Background: Both positive and negative associations between a rare allele of 27-bp repeat polymorphism (eNOS4b/a polymorphism) in intron 4 of endothelial nitric oxide synthase and plasma nitric oxide (NO) concentrations were previously reported. Although these conflicting results were suggested to be partly accounted for smoking status of subjects, no further studies have been accomplished. Methods: We analyzed eNOS4b/a polymorphism in a group of 393 healthy Korean subjects and measured their plasma nitrite and nitrate (NOx) concentrations. NOx concentrations were measured by the Griess method and the genotypes of eNOS4b/a polymorphism determined by the banding pattern on gel electrophoresis. Results: The frequency of eNOS4a allele in this study was 11.6%. The plasma NOx concentrations (in μmol/l) in subjects with eNOS4a allele was found to be significantly higher relative to those in eNOS4b allele (49.68±18.62 and 55.25±20.87, respectively, P<0.05), which was valid only in smokers. Multiple regression analysis revealed that the most predictive contributing factor for plasma NOx concentrations was eNOS4a allele (P<0.01), followed by smoking (P<0.05), total cholesterol (P<0.05), and triglycerides (P<0.05). Conclusion: Our data indicate that there is substantial effect of eNOS4b/a polymorphism on the variance of plasma NOx concentrations in Korean population and that this effect is dependent on smoking status.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalClinica Chimica Acta
Issue number1-2
Publication statusPublished - 2002 Oct

Bibliographical note

Funding Information:
This work was supported in part by Korea National Institute of Health intramural research grant (334-6113-211-207-00) and Science Research Center grant from Korea Science and Engineering Foundation (KOSEF) to the Nitric Oxide Radical Toxicology Research Center (NORTReC) to Dr. Inho Jo, by Biomedical Brain Research Center grant from Ministry of Health and Welfare to Dr. Sangmee Ahn Jo, and by Korea University Institutes of Medical Science grant (2000-n6) to Dr. Chol Shin. We thank Dr. Younjhin Ahn and Mr. Jungbok Lee for their valuable discussions regarding statistical analysis and Ms. Jooyoung Lee for the secretarial assistance.


  • Endothelial nitric oxide synthase gene
  • Korea
  • Plasma nitric oxide concentrations
  • Polymorphism
  • Smoking status

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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