Smooth muscle-like tissues engineered with bone marrow stromal cells

Seung Woo Cho, Il Kwon Kim, Sang Hyun Lim, Dong Ik Kim, Sun Woong Kang, Soo Hyun Kim, Young Ha Kim, Eun Yeol Lee, Cha Yong Choi, Byung Soo Kim

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)


Bone marrow-derived cells have demonstrated the ability to differentiate into multiple mesenchymal cell lineages. Here we tested whether smooth muscle (SM)-like tissues can be created in vivo with bone marrow stromal cells (BMSCs). Cultured canine BMSCs, which expressed SM cell-specific markers including SM α-actin and SM myosin heavy chain, were seeded on three-dimensional, biodegradable polymer scaffolds and implanted into peritoneal cavity of athymic mice. The cell-scaffold constructs retrieved 4 weeks after implantation formed three-dimensional tissues. Immunohistochemical analyses showed that the tissue reconstructs expressed SM α-actin and SM myosin heavy chain. Masson's trichrome staining showed the presence of significant amounts of collagen in the tissue reconstructs. Cells labeled with a fluorescent tracer prior to implantation were still present in the tissue reconstructs 4 weeks after implantation. Non-seeded scaffolds (control groups) retrieved 4 weeks after implantation did not exhibit extensive tissue formation. This study demonstrates the potential of BMSCs as an alternative cell source for tissue engineering of SM.

Original languageEnglish
Pages (from-to)2979-2986
Number of pages8
Issue number15
Publication statusPublished - 2004 Jul

Bibliographical note

Funding Information:
This work was supported by the Korea Ministry of Science and Technology (National Research Laboratory Program, M19911000028-99J0000-02712).


  • Biodegradable polymer scaffold
  • Bone marrow stromal cells
  • Smooth muscle
  • Tissue engineering

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials


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