Solid-phase genotoxicity assay for organic compounds in soil

Renee R. Alexander; Namhyun Chung; Martin Alexander

doi:10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2

Solid-phase genotoxicity assay for organic compounds in soil

Renee R. Alexander, Namhyun Chung, Martin Alexander

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

Original language	English
Pages (from-to)	420-425
Number of pages	6
Journal	Environmental Toxicology and Chemistry
Volume	18
Issue number	3
DOIs	https://doi.org/10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2
Publication status	Published - 1999
Externally published	Yes

Keywords

2-Aminofluorene
Bioassays
Genotoxicants
Pseudomonas putida

ASJC Scopus subject areas

Environmental Chemistry
Health, Toxicology and Mutagenesis

Access to Document

10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2

Cite this

@article{e4edaadff940489aac3ba44957f4b2c1,

title = "Solid-phase genotoxicity assay for organic compounds in soil",

abstract = "A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.",

keywords = "2-Aminofluorene, Bioassays, Genotoxicants, Pseudomonas putida",

author = "Alexander, {Renee R.} and Namhyun Chung and Martin Alexander",

year = "1999",

doi = "10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2",

language = "English",

volume = "18",

pages = "420--425",

journal = "Environmental Toxicology and Chemistry",

issn = "0730-7268",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Solid-phase genotoxicity assay for organic compounds in soil

AU - Alexander, Renee R.

AU - Chung, Namhyun

AU - Alexander, Martin

PY - 1999

Y1 - 1999

N2 - A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

AB - A genotoxicity assay was developed for samples from environments in which toxic organic compounds are largely sorbed. The assay entails measurement of the rate of mutation of a strain of Pseudomonas putida to rifampicin resistance. The ratio of induced to spontaneous mutants was a function of the concentration of a test mutagen in soil. In studies of the utility of the assay in samples amended with 2-aminofluorene as a test mutagen, the ratio of induced to spontaneous mutants declined with time. The decline paralleled the disappearance of extractable 2-aminofluorene from the soil. The ratio of induced to spontaneous mutants also fell in four other soils with dissimilar properties. We suggest that this solid-phase assay is more appropriate for the estimation of genotoxicants sorbed in soil than assays involving extractants or suspensions of soil or sediment samples.

KW - 2-Aminofluorene

KW - Bioassays

KW - Genotoxicants

KW - Pseudomonas putida

UR - http://www.scopus.com/inward/record.url?scp=0033062779&partnerID=8YFLogxK

U2 - 10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2

DO - 10.1897/1551-5028(1999)018<0420:SPGAFO>2.3.CO;2

M3 - Article

AN - SCOPUS:0033062779

SN - 0730-7268

VL - 18

SP - 420

EP - 425

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

IS - 3

ER -

Solid-phase genotoxicity assay for organic compounds in soil

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this