Solubilization of aggregation-prone heterologous proteins by covalent fusion of stress-responsive Escherichia coli protein, SlyD

Kyung Yeon Han, Jong Am Song, Keum Young Ahn, Jin Seung Park, Hyuk Seong Seo, Jeewon Lee

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40 Citations (Scopus)


The proteome profile of Escherichia coli BL21(DE3) generated in response to heat shock stress was analyzed by two-dimensional electrophoresis (2-DE), wherein we identified a FKBP-type peptidyl-prolyl cis-trans isomerse (PPIases), SlyD, as a stress-responsive (i.e. aggregation-resistant) protein. Even under an imposed severe stress condition where 29 out of 858 soluble proteins were totally eliminated and the synthesis levels of 171 proteins decreased over 5-fold, a 3.37-fold increase induced by heat shock treatment was observed in the synthesis level of SlyD compared with a non-stress condition. As a fusion partner, as well as solubility enhancer, SlyD facilitated folding and significantly increased the solubility of many aggregation-prone heterologous proteins in E. coli cytoplasm. SlyD was very effective in sequestering interactive surfaces of heterologous proteins associated with non-specific protein-protein interactions and the formation of inclusion bodies, most likely as a result of intrinsic folding efficiencies and/or chaperone-like activities. SlyD was also shown to be suitable for the production of a biologically active fusion mutant of Pseudomonas putida cutinase that is of considerable biotechnological and commercial interest.

Original languageEnglish
Pages (from-to)543-549
Number of pages7
JournalProtein Engineering, Design and Selection
Issue number11
Publication statusPublished - 2007 Nov

Bibliographical note

Funding Information:
National Research Laboratory Project (ROA-2007-000-20084-0) from the Korea Science and Engineering Foundation (KOSEF); Korean government (MOST), the Korea Health 21 R&D Project (A050750) of the Ministry of Health and Welfare of the Republic of Korea; Second Brain Korea 21 Project; KOSEF grant (R01-2005-000-10355-0); Korea Research Foundation (KRF-2004-041-D00180); Microbial Genomics and Applications Center (Taejon, Republic of Korea).


  • Escherichia coli BL21(DE3)
  • Proteome
  • SlyD
  • Solubility enhancer
  • Stress response

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Molecular Biology


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