Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

Seon Soo Kim, Hyun Jung Byun, Sang Hoon Kim, Han Hyoung Lee, Suk Jun Lee, Sang Joon Kim, Chung Gyu Park, Taehoon Chun

    Research output: Contribution to journalArticlepeer-review

    5 Citations (Scopus)

    Abstract

    Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.

    Original languageEnglish
    Pages (from-to)203-208
    Number of pages6
    JournalBiotechnology letters
    Volume32
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jan

    Bibliographical note

    Funding Information:
    Acknowledgments This work was supported by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project number: A040004).

    Keywords

    • Co-receptor
    • Lymphocyte activation gene-3
    • Mixed lymphocyte reaction
    • Pig
    • T cell activation
    • Xenogeneic response

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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