Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

Seon Soo Kim; Hyun Jung Byun; Sang Hoon Kim; Han Hyoung Lee; Suk Jun Lee; Sang Joon Kim; Chung Gyu Park; Taehoon Chun

doi:10.1007/s10529-009-0144-9

Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

Seon Soo Kim, Hyun Jung Byun, Sang Hoon Kim, Han Hyoung Lee, Suk Jun Lee, Sang Joon Kim, Chung Gyu Park, Taehoon Chun

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.

Original language	English
Pages (from-to)	203-208
Number of pages	6
Journal	Biotechnology letters
Volume	32
Issue number	2
DOIs	https://doi.org/10.1007/s10529-009-0144-9
Publication status	Published - 2010 Jan

Bibliographical note

Funding Information:
Acknowledgments This work was supported by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project number: A040004).

Keywords

Co-receptor
Lymphocyte activation gene-3
Mixed lymphocyte reaction
Pig
T cell activation
Xenogeneic response

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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title = "Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction",

abstract = "Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.",

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author = "Kim, {Seon Soo} and Byun, {Hyun Jung} and Kim, {Sang Hoon} and Lee, {Han Hyoung} and Lee, {Suk Jun} and Kim, {Sang Joon} and Park, {Chung Gyu} and Taehoon Chun",

note = "Funding Information: Acknowledgments This work was supported by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project number: A040004).",

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AU - Byun, Hyun Jung

AU - Kim, Sang Hoon

AU - Lee, Han Hyoung

AU - Lee, Suk Jun

AU - Kim, Sang Joon

AU - Park, Chung Gyu

AU - Chun, Taehoon

N1 - Funding Information: Acknowledgments This work was supported by a grant from the Korea Health 21 R & D Project, Ministry of Health & Welfare, Republic of Korea (Project number: A040004).

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N2 - Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.

AB - Lymphocyte activation gene-3 (LAG-3; CD223) is a transmembrane protein that is structurally similar to CD4. Since LAG-3 has a much higher binding affinity to MHC class II than that of CD4, several approaches using soluble LAG-3 were used to modulate immune responses by activation or inhibition of MHC class II expressing antigen presenting cells. In this study, we constructed soluble pig LAG-3 containing a critical binding site (D1 and D2 region) to MHC class II molecules, combined with a constant region of an immunoglobulin (Ig) heavy chain. Flow cytometry analyses indicated that soluble pig LAG-3 binds to both pig and human MHC class II molecules. Moreover, soluble pig LAG-3 can inhibit human lymphocyte proliferation in the human-pig xenogeneic mixed lymphocyte reaction in a dose-dependent manner. These results indicate that soluble pig LAG-3 may be useful for controlling the xenogeneic T cell immune responses between the human and pig.

KW - Co-receptor

KW - Lymphocyte activation gene-3

KW - Mixed lymphocyte reaction

KW - Pig

KW - T cell activation

KW - Xenogeneic response

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Soluble pig lymphocyte activation gene-3 (LAG-3; CD223) inhibits human-to-pig xenogeneic mixed lymphocyte reaction

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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