Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Inhong Kim; Mijeong Kang; Han Young Woo; Jin Woo Oh; Kwangseuk Kyhm

doi:10.1117/12.2190332

Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Inhong Kim, Mijeong Kang, Han Young Woo, Jin Woo Oh, Kwangseuk Kyhm

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.

Original language	English
Title of host publication	International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015
Editors	Donghyun Kim, Min-Gon Kim, Seung-Han Park
Publisher	SPIE
ISBN (Electronic)	9781628416794
DOIs	https://doi.org/10.1117/12.2190332
Publication status	Published - 2015
Externally published	Yes
Event	2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of Duration: 2015 Feb 25 → 2015 Feb 27

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9523
ISSN (Print)	1605-7422

Conference

Conference	2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015
Country/Territory	Korea, Republic of
City	Jeju
Period	15/2/25 → 15/2/27

Bibliographical note

Publisher Copyright:
© 2015 SPIE.

Keywords

Conjugated polymer
Exciton
Fluorescence quenching
Forster resonance energy transfer (FRET)
Time-resolved fluorescence

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging
Biomaterials

Access to Document

10.1117/12.2190332

Cite this

Kim, I., Kang, M., Woo, H. Y., Oh, J. W., & Kyhm, K. (2015). Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. In D. Kim, M.-G. Kim, & S.-H. Park (Eds.), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015 Article 95230N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9523). SPIE. https://doi.org/10.1117/12.2190332

Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. / Kim, Inhong; Kang, Mijeong; Woo, Han Young et al.
International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. ed. / Donghyun Kim; Min-Gon Kim; Seung-Han Park. SPIE, 2015. 95230N (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9523).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Kim, I, Kang, M, Woo, HY, Oh, JW & Kyhm, K 2015, Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. in D Kim, M-G Kim & S-H Park (eds), International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015., 95230N, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9523, SPIE, 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015, Jeju, Korea, Republic of, 15/2/25. https://doi.org/10.1117/12.2190332

Kim I, Kang M, Woo HY, Oh JW, Kyhm K. Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. In Kim D, Kim MG, Park SH, editors, International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. SPIE. 2015. 95230N. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2190332

Kim, Inhong ; Kang, Mijeong ; Woo, Han Young et al. / Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA. International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015. editor / Donghyun Kim ; Min-Gon Kim ; Seung-Han Park. SPIE, 2015. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

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title = "Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA",

abstract = "We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 {\AA}) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 {\AA} and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.",

keywords = "Conjugated polymer, Exciton, Fluorescence quenching, Forster resonance energy transfer (FRET), Time-resolved fluorescence",

author = "Inhong Kim and Mijeong Kang and Woo, {Han Young} and Oh, {Jin Woo} and Kwangseuk Kyhm",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 ; Conference date: 25-02-2015 Through 27-02-2015",

year = "2015",

doi = "10.1117/12.2190332",

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T1 - Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

AU - Kim, Inhong

AU - Kang, Mijeong

AU - Woo, Han Young

AU - Oh, Jin Woo

AU - Kyhm, Kwangseuk

PY - 2015

Y1 - 2015

N2 - We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.

AB - We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.

KW - Conjugated polymer

KW - Exciton

KW - Fluorescence quenching

KW - Forster resonance energy transfer (FRET)

KW - Time-resolved fluorescence

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DO - 10.1117/12.2190332

M3 - Conference contribution

AN - SCOPUS:84938704414

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015

A2 - Kim, Donghyun

A2 - Kim, Min-Gon

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PB - SPIE

T2 - 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015

Y2 - 25 February 2015 through 27 February 2015

ER -

Solvent-modified ultrafast decay dynamics in conjugated polymer/dye labeled single stranded DNA

Abstract

Publication series

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Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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