Abstract
We have investigated that organic solvent (DMSO, dimethyl sulfoxide) modifies energy transfer efficiency between conjugated polymers (donors) and fluorescein-labeled single stranded DNAs (acceptors). In a mixture of buffer and organic solvent, fluorescence of the acceptors is significantly enhanced compared to that of pure water solution. This result can be attributed to change of the donor-Acceptor environment such as decreased hydrophobicity of polymers, screening effect of organic solvent molecules, resulting in an enhanced energy transfer efficiency. Time-resolved fluorescence decay of the donors and the acceptors was modelled by considering the competition between the energy harvesting Foerster resonance energy transfer and the energy-wasting quenching. This enables to quantity that the Foerster distance (R0 = 43.3 Å) and resonance energy transfer efficiency (EFRET = 58.7 %) of pure buffer solution become R0 = 38.6 Å and EFRET = 48.0 % when 80% DMSO/buffer mixture is added.
Original language | English |
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Title of host publication | International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy 2015, NBSIS 2015 |
Editors | Donghyun Kim, Min-Gon Kim, Seung-Han Park |
Publisher | SPIE |
ISBN (Electronic) | 9781628416794 |
DOIs | |
Publication status | Published - 2015 |
Externally published | Yes |
Event | 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 - Jeju, Korea, Republic of Duration: 2015 Feb 25 → 2015 Feb 27 |
Publication series
Name | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
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Volume | 9523 |
ISSN (Print) | 1605-7422 |
Conference
Conference | 2nd International Conference on Nano-Bio Sensing, Imaging, and Spectroscopy, NBSIS 2015 |
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Country/Territory | Korea, Republic of |
City | Jeju |
Period | 15/2/25 → 15/2/27 |
Bibliographical note
Publisher Copyright:© 2015 SPIE.
Keywords
- Conjugated polymer
- Exciton
- Fluorescence quenching
- Forster resonance energy transfer (FRET)
- Time-resolved fluorescence
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Radiology Nuclear Medicine and imaging
- Biomaterials