Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice

Dae Won Kim; Ki Yeon Yoo; Yoon Bok Lee; Keun Ha Lee; Heon Soo Sohn; Sung Joon Lee; Kyung Hwan Cho; Yong Kook Shin; In Koo Hwang; Moo Ho Won; Dong Woo Kim

doi:10.1089/jmf.2008.1027

Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice

Dae Won Kim, Ki Yeon Yoo, Yoon Bok Lee, Keun Ha Lee, Heon Soo Sohn, Sung Joon Lee, Kyung Hwan Cho, Yong Kook Shin, In Koo Hwang, Moo Ho Won, Dong Woo Kim

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14 Citations (Scopus)

Abstract

We evaluated the protective effects of soy isoflavones (SIF) against osteoporosis in middle-aged ovariectomized (OVX) mice. SIF (30mg/kg or 60>mg/kg) or 17β-estradiol (E₂) was administered to OVX mice for 4 months after bilateral ovariectomy. We observed the biochemical markers of bone turnover, e.g., alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), in serum. We also observed the bone mineral density (BMD) in femurs and lumbar vertebrae. In addition, we examined trabecular bone and interstitial cells in the femur using hematoxylin and eosin staining. The decrease in ALP levels and the increase in TRAP levels normally resulting from ovariectomy were suppressed by administration of 60mg/kg SIF or E₂. Administration of 60>mg/kg SIF or E₂ also maintained the BMD, trabecular bone, and interstitial cells in OVX mice compared to those in pre-OVX mice. These results suggest that 60mg/kg SIF effectively mitigates ovariectomy-induced osteoporosis in middle-aged mice.

Original language	English
Pages (from-to)	536-541
Number of pages	6
Journal	Journal of Medicinal Food
Volume	12
Issue number	3
DOIs	https://doi.org/10.1089/jmf.2008.1027
Publication status	Published - 2009 Jun 1

Keywords

Alkaline phosphatase
Bone mineral density
Mice
Ovariectomy
Soy isoflavones
Tartrate-resistant acid phosphatase

ASJC Scopus subject areas

Medicine (miscellaneous)
Nutrition and Dietetics

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10.1089/jmf.2008.1027

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title = "Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice",

abstract = "We evaluated the protective effects of soy isoflavones (SIF) against osteoporosis in middle-aged ovariectomized (OVX) mice. SIF (30mg/kg or 60>mg/kg) or 17β-estradiol (E2) was administered to OVX mice for 4 months after bilateral ovariectomy. We observed the biochemical markers of bone turnover, e.g., alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP), in serum. We also observed the bone mineral density (BMD) in femurs and lumbar vertebrae. In addition, we examined trabecular bone and interstitial cells in the femur using hematoxylin and eosin staining. The decrease in ALP levels and the increase in TRAP levels normally resulting from ovariectomy were suppressed by administration of 60mg/kg SIF or E2. Administration of 60>mg/kg SIF or E2 also maintained the BMD, trabecular bone, and interstitial cells in OVX mice compared to those in pre-OVX mice. These results suggest that 60mg/kg SIF effectively mitigates ovariectomy-induced osteoporosis in middle-aged mice.",

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author = "Kim, {Dae Won} and Yoo, {Ki Yeon} and Lee, {Yoon Bok} and Lee, {Keun Ha} and Sohn, {Heon Soo} and Lee, {Sung Joon} and Cho, {Kyung Hwan} and Shin, {Yong Kook} and Hwang, {In Koo} and Won, {Moo Ho} and Kim, {Dong Woo}",

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AU - Lee, Keun Ha

AU - Sohn, Heon Soo

AU - Lee, Sung Joon

AU - Cho, Kyung Hwan

AU - Shin, Yong Kook

AU - Hwang, In Koo

AU - Won, Moo Ho

AU - Kim, Dong Woo

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Soy isoflavones mitigate long-term femoral and lumbar vertebral bone loss in middle-aged ovariectomized mice

Abstract

Keywords

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