TY - JOUR
T1 - Spatial learning enhances the expression of inositol 1,4,5-trisphosphate 3-kinase A in the hippocampal formation of rat
AU - Kim, Il Hwan
AU - Park, Soon Kwon
AU - Sun, Woong
AU - Kang, Yunhee
AU - Kim, Hyun Taek
AU - Kim, Hyun
N1 - Funding Information:
We thank Dr. Sangduk Kim for valuable comments on the manuscript and Mr. J. Jung for assistants. This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (02-PJ1-PG1-CH06-0001).
PY - 2004/4/29
Y1 - 2004/4/29
N2 - Calcium-mediated signaling is crucial for the synaptic plasticity and long-term memory storage, which requires de novo protein synthesis. Inositol 1,4,5-trisphosphate 3-kinase A (IP3K-A) is an enzyme, which is involved in the maintenance of intracellular calcium homeostasis by converting inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5- tetrakisphosphate (IP4). Because IP3K-A is enriched in the dendritic spines of hippocampal neurons, it has been speculated that this enzyme is involved in the memory formation. In the present study, we demonstrated that the expression of IP3K-A is increased in the hippocampal formation of the rats during the Morris water maze training. Immunohistochemical analysis indicated the specific induction of IP 3K-A protein in the hippocampal formation following 5-day water maze training. Furthermore, in situ hybridization histochemistry showed that the induction of IP3K-A mRNA in the hippocampal formation was observed on the first day of training, and the induced level of IP3K-A mRNA was maintained until the fifth day of training. These results suggest that IP 3K-A plays a role in the processing of spatial memory, most likely by regulating the calcium signaling in the dendritic spines of hippocampal formation.
AB - Calcium-mediated signaling is crucial for the synaptic plasticity and long-term memory storage, which requires de novo protein synthesis. Inositol 1,4,5-trisphosphate 3-kinase A (IP3K-A) is an enzyme, which is involved in the maintenance of intracellular calcium homeostasis by converting inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5- tetrakisphosphate (IP4). Because IP3K-A is enriched in the dendritic spines of hippocampal neurons, it has been speculated that this enzyme is involved in the memory formation. In the present study, we demonstrated that the expression of IP3K-A is increased in the hippocampal formation of the rats during the Morris water maze training. Immunohistochemical analysis indicated the specific induction of IP 3K-A protein in the hippocampal formation following 5-day water maze training. Furthermore, in situ hybridization histochemistry showed that the induction of IP3K-A mRNA in the hippocampal formation was observed on the first day of training, and the induced level of IP3K-A mRNA was maintained until the fifth day of training. These results suggest that IP 3K-A plays a role in the processing of spatial memory, most likely by regulating the calcium signaling in the dendritic spines of hippocampal formation.
KW - Hippocampal formation
KW - Inositol 1,4,5-trisphosphate 3-kinase A
KW - Learning and memory: systems and functions - animals
KW - Neural basis of behavior
KW - Spatial learning
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U2 - 10.1016/j.molbrainres.2003.12.016
DO - 10.1016/j.molbrainres.2003.12.016
M3 - Article
C2 - 15093681
AN - SCOPUS:1942485771
SN - 0006-8993
VL - 124
SP - 12
EP - 19
JO - Brain Research
JF - Brain Research
IS - 1
ER -