Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip

Yeon Seok Kim, Javed H. Niazi, Man Bock Gu

    Research output: Contribution to journalArticlepeer-review

    112 Citations (Scopus)

    Abstract

    An electrochemical sensing system for oxytetracycline (OTC) detection was developed using ssDNA aptamer immobilized on gold interdigitated array (IDA) electrode chip. A highly specific ssDNA aptamer that bind to OTC with high affinity was employed to discriminate other tetracyclines (TCs), such as doxycycline (DOX) and tetracycline (TET). The immobilized thiol-modified aptamer on gold electrode chip served as a biorecognition element for the target molecules and the electrochemical signals generated from interactions between the aptamers and the target molecules was evaluated by cyclic voltammetry (CV) and square wave voltammetry (SWV). The current decrease due to the interference of bound OTC, DOX or TET was analyzed with the electron flow produced by a redox reaction between ferro- and ferricyanide. The specificity of developed EC-biosensor for OTC was highly distinguishable from the structurally similar antibiotics (DOX and TET). The dynamic range was determined to be 1-100 nM of OTC concentration in semi-logarithmic coordinates.

    Original languageEnglish
    Pages (from-to)250-254
    Number of pages5
    JournalAnalytica Chimica Acta
    Volume634
    Issue number2
    DOIs
    Publication statusPublished - 2009 Feb 23

    Keywords

    • Aptamer
    • Electrochemical detection
    • Interdigitated array electrode
    • Oxytetracycline

    ASJC Scopus subject areas

    • Analytical Chemistry
    • Biochemistry
    • Environmental Chemistry
    • Spectroscopy

    Fingerprint

    Dive into the research topics of 'Specific detection of oxytetracycline using DNA aptamer-immobilized interdigitated array electrode chip'. Together they form a unique fingerprint.

    Cite this