Speckle-field digital holographic microscopy

Yong Keun Park; Wonshik Choi; Zahid Yaqoob; Ramachandra Dasari; Kamran Badizadegan; Michael S. Feld

doi:10.1117/12.842799

Speckle-field digital holographic microscopy

Yong Keun Park^*, Wonshik Choi, Zahid Yaqoob, Ramachandra Dasari, Kamran Badizadegan, Michael S. Feld

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs	https://doi.org/10.1117/12.842799
Publication status	Published - 2010
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States Duration: 2010 Jan 23 → 2010 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7568
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/Territory	United States
City	San Francisco, CA
Period	10/1/23 → 10/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.842799

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Park, YK, Choi, W, Yaqoob, Z, Dasari, R, Badizadegan, K & Feld, MS 2010, Speckle-field digital holographic microscopy. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 75681O, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.842799

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author = "Park, \{Yong Keun\} and Wonshik Choi and Zahid Yaqoob and Ramachandra Dasari and Kamran Badizadegan and Feld, \{Michael S.\}",

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AU - Park, Yong Keun

AU - Choi, Wonshik

AU - Yaqoob, Zahid

AU - Dasari, Ramachandra

AU - Badizadegan, Kamran

AU - Feld, Michael S.

PY - 2010

Y1 - 2010

N2 - We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

AB - We report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging (For the detail, please see the Ref. [1]). A speckle beam of a complex spatial pattern is used for illumination to reduce fixed pattern noise and to improve optical sectioning capability. By recording of the electric field of speckle, we demonstrate high contrast 3D live cell imaging without the need for axial scanning - neither objective lens nor sample stage. This technique has great potential in studying biological samples with improved sensitivity, resolution and optical sectioning capability.

UR - https://www.scopus.com/pages/publications/77951809861

U2 - 10.1117/12.842799

DO - 10.1117/12.842799

M3 - Conference contribution

AN - SCOPUS:77951809861

SN - 9780819479648

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

Y2 - 23 January 2010 through 25 January 2010

ER -

Speckle-field digital holographic microscopy

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