Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Yong Keun Park; Toyohiko Yamauchi; Wonshik Choi; Ramachandra Dasari; Michael S. Feld

doi:10.1364/OL.34.003668

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Yong Keun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, Michael S. Feld

Department of Physics

Research output: Contribution to journal › Article › peer-review

164 Citations (Scopus)

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

Original language	English
Pages (from-to)	3668-3670
Number of pages	3
Journal	Optics Letters
Volume	34
Issue number	23
DOIs	https://doi.org/10.1364/OL.34.003668
Publication status	Published - 2009 Dec 1

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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AU - Yamauchi, Toyohiko

AU - Choi, Wonshik

AU - Dasari, Ramachandra

AU - Feld, Michael S.

PY - 2009/12/1

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AB - We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual human red blood cells. The end point of this study is noninvasive monitoring of physiological states of intact living cells.

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Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Abstract

ASJC Scopus subject areas

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