Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Yong Keun Park; Toyohiko Yamauchi; Wonshik Choi; Ramachandra Dasari; Michael S. Feld

doi:10.1117/12.842813

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

Yong Keun Park, Toyohiko Yamauchi, Wonshik Choi, Ramachandra Dasari, Michael S. Feld

Department of Physics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.

Original language	English
Title of host publication	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
DOIs	https://doi.org/10.1117/12.842813
Publication status	Published - 2010
Event	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII - San Francisco, CA, United States Duration: 2010 Jan 23 → 2010 Jan 25

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	7568
ISSN (Print)	1605-7422

Other

Other	Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII
Country/Territory	United States
City	San Francisco, CA
Period	10/1/23 → 10/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1117/12.842813

Cite this

Park, Y. K., Yamauchi, T., Choi, W., Dasari, R., & Feld, M. S. (2010). Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. In Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII Article 756812 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568). https://doi.org/10.1117/12.842813

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. / Park, Yong Keun; Yamauchi, Toyohiko; Choi, Wonshik et al.
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII. 2010. 756812 (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7568).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Park, YK, Yamauchi, T, Choi, W, Dasari, R & Feld, MS 2010, Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells. in Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII., 756812, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, San Francisco, CA, United States, 10/1/23. https://doi.org/10.1117/12.842813

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abstract = "We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.",

author = "Park, {Yong Keun} and Toyohiko Yamauchi and Wonshik Choi and Ramachandra Dasari and Feld, {Michael S.}",

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AU - Feld, Michael S.

PY - 2010

Y1 - 2010

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AB - We report a practical method for label-free quantification of specific molecules using spectroscopic imaging of sampleinduced phase shifts (for the detail, please see the Ref. [1]). Diffraction phase microscopy equipped with various wavelengths of light source is used to record wavelength-dependent phase images. We first perform dispersion measurements on pure solutions of single molecular species present in the cells, such as albumin and hemoglobin (Hb). With this prior calibration of molecular specific dispersion, we demonstrate the extraction of Hb concentration from individual red blood cells (RBCs). The end point of this study is non-invasive monitoring of physiological states of intact living cells.

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M3 - Conference contribution

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T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

T2 - Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII

Y2 - 23 January 2010 through 25 January 2010

ER -

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

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