Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2

Jong Ho Kim; I. Rang Lim; Hyung Joon Joo; Seung Cheol Choi; Ji Hyun Choi; Long Hui Cui; Lisa Im; Soon Jun Hong; Do Sun Lim

doi:10.1016/j.bbrc.2015.10.083

Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2

Jong Ho Kim, I. Rang Lim, Hyung Joon Joo, Seung Cheol Choi, Ji Hyun Choi, Long Hui Cui, Lisa Im, Soon Jun Hong, Do Sun Lim

Department of Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, VWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.

Original language	English
Pages (from-to)	372-379
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	468
Issue number	1-2
DOIs	https://doi.org/10.1016/j.bbrc.2015.10.083
Publication status	Published - 2015 Dec 4

Bibliographical note

Funding Information:
We thank Chi-Yeon Park (Korea University) and Sangseok Yun (KAIST) for their technical assistance. This research was supported by a grant of the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( NRF-2011-0012013 ) and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare ( A120275 ), Republic of Korea.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.

Keywords

Adipose stem cell
Fibroblast growth factor 2
Poly-2-hydroxyethyl methacrylate
Sphere
in vitro angiogenesis

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2015.10.083

Cite this

Kim, J. H., Lim, I. R., Joo, H. J., Choi, S. C., Choi, J. H., Cui, L. H., Im, L., Hong, S. J., & Lim, D. S. (2015). Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2. Biochemical and biophysical research communications, 468(1-2), 372-379. https://doi.org/10.1016/j.bbrc.2015.10.083

Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2. / Kim, Jong Ho; Lim, I. Rang; Joo, Hyung Joon et al.
In: Biochemical and biophysical research communications, Vol. 468, No. 1-2, 04.12.2015, p. 372-379.

Research output: Contribution to journal › Article › peer-review

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abstract = "A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, VWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.",

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note = "Funding Information: We thank Chi-Yeon Park (Korea University) and Sangseok Yun (KAIST) for their technical assistance. This research was supported by a grant of the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( NRF-2011-0012013 ) and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare ( A120275 ), Republic of Korea. Publisher Copyright: {\textcopyright} 2015 Elsevier Inc. All rights reserved.",

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AU - Choi, Seung Cheol

AU - Choi, Ji Hyun

AU - Cui, Long Hui

AU - Im, Lisa

AU - Hong, Soon Jun

AU - Lim, Do Sun

N1 - Funding Information: We thank Chi-Yeon Park (Korea University) and Sangseok Yun (KAIST) for their technical assistance. This research was supported by a grant of the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( NRF-2011-0012013 ) and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare ( A120275 ), Republic of Korea. Publisher Copyright: © 2015 Elsevier Inc. All rights reserved.

PY - 2015/12/4

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N2 - A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, VWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.

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Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2

Abstract

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Keywords

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