Sphere formation of adipose stem cell engineered by poly-2-hydroxyethyl methacrylate induces in vitro angiogenesis through fibroblast growth factor 2

Jong Ho Kim, I. Rang Lim, Hyung Joon Joo, Seung Cheol Choi, Ji Hyun Choi, Long Hui Cui, Lisa Im, Soon Jun Hong, Do Sun Lim

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16 Citations (Scopus)


A number of researchers have been reporting a wide range of in vitro and in vivo studies of cell engraftment to enhance angiogenesis using stem cells. Despite these efforts, studies involving three-dimensional (3D) culture method that mimics in vivo environment have not reached its peak yet. In this study, we investigated the change and effects on cellular angiogenic growth factors through sphere formation of adipose stem cell (ASC) which is engineered by poly-2-hydroxyethyl methacrylate (Poly-HEMA). First of all, we successfully induced sphere formation of ASC (sph-ASC) on Poly-HEMA coated plates. sph-ASC represented significantly higher expression levels of anti-apoptotic and hypoxic factors compared to monolayer adherent ASC (adh-ASC). Interestingly, sph-ASC showed higher mRNA levels of the following genes; CD31, CD144, VWF, IGF-2, MCP-1, PDGF-A, VEGF-A, VEGF-C, and FGF-2. In addition, mRNA expressions of angiogenic growth factor receptors such as Flk1, FGFR1, FGFR2, and Tie2 were elevated in sph-ASC. In protein level, Cytokine/Chemokines antibody array revealed a significant increase of FGF-2 in sph-ASC (3.17-fold) compared to adh-ASC. To investigate the effects of FGF-2 on sph-ASC, Matrigel angiogenic invasion assay showed significant reduced level of FGF-2 in FGF-2 siRNA transfected sph-ASC (2.27-fold) compared to negative control siRNA transfected sph-ASC. These findings suggest that Poly-HEMA coated plates induce sphere formation of ASC which has significantly higher expression of FGF-2, and plays a critical role as a major regulating growth factor of in vitro angiogenesis.

Original languageEnglish
Pages (from-to)372-379
Number of pages8
JournalBiochemical and biophysical research communications
Issue number1-2
Publication statusPublished - 2015 Dec 4

Bibliographical note

Funding Information:
We thank Chi-Yeon Park (Korea University) and Sangseok Yun (KAIST) for their technical assistance. This research was supported by a grant of the Basic Science Research Program through the National Research Foundation of Korea (NRF) , funded by the Ministry of Education ( NRF-2011-0012013 ) and a grant of the Korea Healthcare technology R&D Project, Ministry of Health & Welfare ( A120275 ), Republic of Korea.

Publisher Copyright:
© 2015 Elsevier Inc. All rights reserved.


  • Adipose stem cell
  • Fibroblast growth factor 2
  • Poly-2-hydroxyethyl methacrylate
  • Sphere
  • in vitro angiogenesis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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