TY - JOUR
T1 - Spheroid formation of hepatocarcinoma cells in microwells
T2 - Experiments and Monte Carlo simulations
AU - Wang, Yan
AU - Kim, Myung Hee
AU - Tabaei, Seyed R.
AU - Park, Jae Hyeok
AU - Na, Kyuhwan
AU - Chung, Seok
AU - Zhdanov, Vladimir P.
AU - Cho, Nam Joon
N1 - Funding Information:
This work was supported by National Research Foundation of Singapore.
Publisher Copyright:
© 2016 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2016/8
Y1 - 2016/8
N2 - The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated lowadhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.
AB - The formation of spherical aggregates during the growth of cell population has long been observed under various conditions. We observed the formation of such aggregates during proliferation of Huh-7.5 cells, a human hepatocarcinoma cell line, in a microfabricated lowadhesion microwell system (SpheroFilm; formed of mass-producible silicone elastomer) on the length scales up to 500 μm. The cell proliferation was also tracked with immunofluorescence staining of F-actin and cell proliferation marker Ki-67. Meanwhile, our complementary 3D Monte Carlo simulations, taking cell diffusion and division, cell-cell and cell-scaffold adhesion, and gravity into account, illustrate the role of these factors in the formation of spheroids. Taken together, our experimental and simulation results provide an integrative view of the process of spheroid formation for Huh-7.5 cells.
UR - http://www.scopus.com/inward/record.url?scp=84991501106&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0161915
DO - 10.1371/journal.pone.0161915
M3 - Article
C2 - 27571565
AN - SCOPUS:84991501106
SN - 1932-6203
VL - 11
JO - PLoS One
JF - PLoS One
IS - 8
M1 - e0161915
ER -