Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides

Sang Jun Sim; Jaemin Joun; Min Eui Hong; Anil Kumar Patel

doi:10.1016/j.biortech.2019.121820

Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides

Sang Jun Sim, Jaemin Joun, Min Eui Hong, Anil Kumar Patel

Research output: Contribution to journal › Article › peer-review

62 Citations (Scopus)

Abstract

Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O₂-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.

Original language	English
Article number	121820
Journal	Bioresource technology
Volume	291
DOIs	https://doi.org/10.1016/j.biortech.2019.121820
Publication status	Published - 2019 Nov

Bibliographical note

Funding Information:
The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government ( Ministry of Science and ICT ) in 2017 (grant number: KCRC-2014M1A8A1049278 ) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2019R1A2C3009821) and Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ).

Publisher Copyright:
© 2019 Elsevier Ltd

Keywords

Energy
Lipid
Microalgae
SMCS
Split mixotrophy

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2019.121820

Cite this

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title = "Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides",

abstract = "Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O2-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.",

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author = "Sim, {Sang Jun} and Jaemin Joun and Hong, {Min Eui} and Patel, {Anil Kumar}",

note = "Funding Information: The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government ( Ministry of Science and ICT ) in 2017 (grant number: KCRC-2014M1A8A1049278 ) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2019R1A2C3009821) and Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ). Publisher Copyright: {\textcopyright} 2019 Elsevier Ltd",

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AU - Hong, Min Eui

AU - Patel, Anil Kumar

N1 - Funding Information: The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government ( Ministry of Science and ICT ) in 2017 (grant number: KCRC-2014M1A8A1049278 ) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2019R1A2C3009821) and Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ). Publisher Copyright: © 2019 Elsevier Ltd

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N2 - Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O2-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.

AB - Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O2-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.

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Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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