Split mixotrophy: A novel cultivation strategy to enhance the mixotrophic biomass and lipid yields of Chlorella protothecoides

Sang Jun Sim, Jaemin Joun, Min Eui Hong, Anil Kumar Patel

    Research output: Contribution to journalArticlepeer-review

    62 Citations (Scopus)

    Abstract

    Mixotrophy (M) assumes sum of autotrophic (A) and heterotrophic (H) growths. In this study, a novel split-mixotrophic cultivation strategy (SMCS) developed as better mixotrophy via offering mutual-benefits through gas-exchange at both headspaces while splitting both trophic modes. To quantify synergistic-growth effects in combined-autotrophy and combined-heterotrophy (CA&CH) of SMCS, gross O2-evolution, DIC and DO concentrations were compared with A, H and M. Average 12–14% and 26–32% increase in DIC and DO concentrations were determined respectively in CA and CH than A, H and M. Biomass yield in CA + CH was increased approx.1.5-folds higher than yields of A + H and M regimes. These results show SMCS as better cultivation strategy than the M by increased biomass and lipid yields. Challenges associated with organic carbon can be solved by SMCS viz. chlorophyll loss, organic carbon uptake inhibition. SMCS could be a breakthrough to integrate bacterial process with algae for better bioprocess economy and energy recovery.

    Original languageEnglish
    Article number121820
    JournalBioresource technology
    Volume291
    DOIs
    Publication statusPublished - 2019 Nov

    Bibliographical note

    Funding Information:
    The authors would like to acknowledge the support of the Korea CCS R&D Center (Korea CCS 2020 Project) funded by the Korea government ( Ministry of Science and ICT ) in 2017 (grant number: KCRC-2014M1A8A1049278 ) and National Research Foundation of Korea (NRF) funded by the Korea government (grant number: NRF-2019R1A2C3009821) and Korea Institute of Energy Technology Evaluation and Planning ( KETEP ) grant funded by the Korea government ( Ministry of Trade, Industry and Energy ) (grant number: 20172010202050 ).

    Publisher Copyright:
    © 2019 Elsevier Ltd

    Keywords

    • Energy
    • Lipid
    • Microalgae
    • SMCS
    • Split mixotrophy

    ASJC Scopus subject areas

    • Bioengineering
    • Environmental Engineering
    • Renewable Energy, Sustainability and the Environment
    • Waste Management and Disposal

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