Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages

Seung Yoon Park, Mi Yeon Jung, Sung Jin Lee, Kae Bok Kang, Alexei Gratchev, Vladimir Riabov, Julia Kzhyshkowska, In San Kim

Research output: Contribution to journalArticlepeer-review

105 Citations (Scopus)


Stabilin-1 is specifically expressed in alternatively activated macrophages. These macrophages participate in anti-inflammatory and healing processes, and display a high phagocytic capacity. In this study, we provide evidence that stabilin-1 is a membrane receptor that performs a crucial function in the clearance of cell corpses. Stabilin-1 is expressed on the cell surface of alternatively activated macrophages and is recruited to the sites of recognition and engulfment of apoptotic bodies, as well as to early phagosomes. Blocking stabilin-1 in macrophages results in defective engulfment of aged red blood cells. Ectopic expression of stabilin-1 induces the binding and engulfment of aged cells in mouse fibroblast L cells. The binding and phagocytosis are dependent on phosphatidylserine (PS), which is well known as an engulfing ligand. Furthermore, using PS-coated beads, we demonstrate that PS directly interacts with stabilin-1 and is sufficient for stabilin-1-mediated phagocytosis. EGF-like domain repeat in stabilin-1 is responsible for PS recognition and binding. Thus, our results demonstrate that stabilin-1, found on alternatively activated macrophages, is a phagocytic receptor mediating the clearance of apoptotic cells in a PS-dependent manner. Therefore, this protein might play an important role in the maintenance of tissue homeostasis and prevention of autoimmunity.

Original languageEnglish
Pages (from-to)3365-3373
Number of pages9
JournalJournal of Cell Science
Issue number18
Publication statusPublished - 2009 Sept 15
Externally publishedYes


  • Aged RBC
  • Apoptotic cells
  • Macrophages
  • Phagocytosis
  • Stabilin-1

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Stabilin-1 mediates phosphatidylserine-dependent clearance of cell cropses in alternatively activated macrophages'. Together they form a unique fingerprint.

Cite this