Stabilized magnetic enzyme aggregates on graphene oxide for high performance phenol and bisphenol A removal

Na Liu, Gang Liang, Xinwei Dong, Xiaoli Qi, Jungbae Kim, Yunxian Piao

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)


We develop a magnetically-separable and stabilized method for the remediation of phenol and bisphenol A in water by the biocatalysis of magnetic enzyme aggregates on graphene oxide (GRO). Both magnetic nanoparticles (MNPs) and tyrosinase (Tyr) were covalently attached on GRO (Mag-CA-Tyr/GRO), and additional glutaraldehyde treatment was performed to construct multi-layers of cross-linked MNPs and Tyr aggregates on GRO (Mag-EC-Tyr/GRO). Mag-EC-Tyr/GRO consisted of thicker layers than the covalent attached method according to atomic force microscopy analysis, and degraded both phenol and bisphenol A in high efficiency. Over broad ranges of temperature and pH, the Mag-EC-Tyr/GRO degraded phenol more efficiently than free and covalent attached Tyr. Mag-EC-Tyr/GRO was more stable than the covalent attached Tyr, and retained over 56% of its initial activity after five cycles of repeated uses for phenol degradation. Owning to the high stability and robustness property, Mag-EC-Tyr/GRO enabled successful degradation of bisphenol A in the environmental water.

Original languageEnglish
Pages (from-to)1026-1034
Number of pages9
JournalChemical Engineering Journal
Publication statusPublished - 2016 Dec 15

Bibliographical note

Funding Information:
This work was financially supported by the Science and Technology Development Program of Jilin Province , China ( 20150101094JC ).


  • Bisphenol A
  • Graphene oxide
  • Magnetic separation
  • Phenol
  • Tyrosinase

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry
  • Chemical Engineering(all)
  • Industrial and Manufacturing Engineering


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