Stable and continuous long-term enzymatic reaction using an enzyme-nanofiber composite

Jin Hyung Lee, Ee Taek Hwang, Byoung Chan Kim, Sun Mi Lee, Byoung In Sang, Yong Su Choi, Jungbae Kim, Man Bock Gu

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43 Citations (Scopus)


This study shows the preparation and application of enzyme-nanofiber composites for long-term stable operation. The enzyme-nanofiber composite was prepared by coating an enzyme aggregate, the esterase from Rhizopus oryzae, on the surface of the nanofibers. After immobilization on the nanofiber, the apparent Km for the immobilized esterase was 1.48-fold higher than that of the free esterase, with values of 0.98 and 1.35 mM for the free and immobilized enzymes, respectively. It was found that enzyme-nanofiber was very stable, even when the fibers were shaken in glass vials, preserving 80% of the initial activity for 100 days. In addition, the enzyme-nanofiber composite was used repeatedly in 30 cycles of substrate hydrolysis and still remained active. Consequently, the esterase-nanofiber composite was employed within a continuous reactor system to evaluate its use in a long-term and stable continuous substrate hydrolysis reaction. It was found that the production of p-nitrophenol was stable for at least 400 h. This study demonstrates that the enzyme-nanofiber composite can be used in both repeated-batch mode and a continuous mode for a long-term stable operation.

Original languageEnglish
Pages (from-to)1301-1307
Number of pages7
JournalApplied Microbiology and Biotechnology
Issue number6
Publication statusPublished - 2007 Jul


  • Continuous reactor
  • Enzyme immobilization
  • Enzyme reactor
  • Enzyme stabilization
  • Esterase
  • Nanofiber

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology


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