Abstract
This study shows the preparation and application of enzyme-nanofiber composites for long-term stable operation. The enzyme-nanofiber composite was prepared by coating an enzyme aggregate, the esterase from Rhizopus oryzae, on the surface of the nanofibers. After immobilization on the nanofiber, the apparent Km for the immobilized esterase was 1.48-fold higher than that of the free esterase, with values of 0.98 and 1.35 mM for the free and immobilized enzymes, respectively. It was found that enzyme-nanofiber was very stable, even when the fibers were shaken in glass vials, preserving 80% of the initial activity for 100 days. In addition, the enzyme-nanofiber composite was used repeatedly in 30 cycles of substrate hydrolysis and still remained active. Consequently, the esterase-nanofiber composite was employed within a continuous reactor system to evaluate its use in a long-term and stable continuous substrate hydrolysis reaction. It was found that the production of p-nitrophenol was stable for at least 400 h. This study demonstrates that the enzyme-nanofiber composite can be used in both repeated-batch mode and a continuous mode for a long-term stable operation.
Original language | English |
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Pages (from-to) | 1301-1307 |
Number of pages | 7 |
Journal | Applied Microbiology and Biotechnology |
Volume | 75 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2007 Jul |
Keywords
- Continuous reactor
- Enzyme immobilization
- Enzyme reactor
- Enzyme stabilization
- Esterase
- Nanofiber
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology