STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING

Jeongsoo Hur; Aram Chung

STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (10⁶ cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.

Original language	English
Title of host publication	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Pages	515-516
Number of pages	2
ISBN (Electronic)	9781733419031
Publication status	Published - 2021
Event	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021 - Palm Springs, Virtual, United States Duration: 2021 Oct 10 → 2021 Oct 14

Publication series

Name	MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference	25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021
Country/Territory	United States
City	Palm Springs, Virtual
Period	21/10/10 → 21/10/14

Bibliographical note

Funding Information:
This work was supported by the Samsung Research Funding and Incubation Center for Future Technology (GrantNo.SRFC-IT1802-03).PrimaryhumanT-cellswerepreparedandhandledwiththeinstitution’sInstitutional ReviewBoard(IRB)approval(#202101091).

Publisher Copyright:
© 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Keywords

CAR-T Immunotherapy
Gene Delivery
Intracellular Delivery
Microfluidics
T-Cell Engineering

ASJC Scopus subject areas

Bioengineering
Chemical Engineering (miscellaneous)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

Hur, J., & Chung, A. (2021). STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 515-516). (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING. / Hur, Jeongsoo; Chung, Aram.
MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. p. 515-516 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hur, J & Chung, A 2021, STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING. in MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 515-516, 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, Virtual, United States, 21/10/10.

Hur J, Chung A. STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING. In MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2021. p. 515-516. (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Hur, Jeongsoo ; Chung, Aram. / STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING. MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2021. pp. 515-516 (MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

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abstract = "A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (106 cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.",

keywords = "CAR-T Immunotherapy, Gene Delivery, Intracellular Delivery, Microfluidics, T-Cell Engineering",

author = "Jeongsoo Hur and Aram Chung",

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AB - A novel microfluidic approach specifically designed to engineer human primary T-lymphocytes effectively with high scalability while maintaining cell functionality is reported. The system employs a unique cell elongation-restoration phenomenon called “cell stretching,” which enables highly effective intracellular delivery of external cargos into cells. Vortical recirculation flows are exerted where cells are hydrodynamically elongated and restored, resulting in effective permeabilization of the cellular membrane. Using the platform, highly efficient (>90%), low-material-cost (<$1), minimally invasive, and high-throughput (106 cells/min) delivery of various cargos into human primary T-cells was achieved, demonstrating the practical utility for chimeric antigen receptor (CAR) T-cell-based cancer immunotherapy.

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STABLE AND SCALABLE ENGINEERING OF HUMAN PRIMARY T CELLS VIA MICROFLUIDIC CELL STRETCHING

Abstract

Publication series

Conference

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Other files and links

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Cite this