We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.
Bibliographical noteFunding Information:
This research was supported by the National Institutes of Health (P41-RR02594-18). Park was supported by a Samsung Scholarship. Diez-Silva and Suresh acknowledge support from the Interdisciplinary Research Group on Infectious Diseases, which is supported by the Singapore-MIT Alliance for Research and Technology and by the National Institutes of Health (1R01HL094270-01A1).
- Electric field
- Light scattering
- Plasmodium falciparum
- Red blood cell
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering