Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

Yong Keun Park; Monica Diez-Silva; Dan Fu; Gabriel Popescu; Wonshik Choi; Ishan Barman; Subra Suresh; Michael S. Feld

doi:10.1117/1.3369966

Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

Yong Keun Park, Monica Diez-Silva, Dan Fu, Gabriel Popescu, Wonshik Choi, Ishan Barman, Subra Suresh, Michael S. Feld

Department of Physics

Research output: Contribution to journal › Article › peer-review

92 Citations (Scopus)

Abstract

We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.

Original language	English
Article number	020506
Journal	Journal of biomedical optics
Volume	15
Issue number	2
DOIs	https://doi.org/10.1117/1.3369966
Publication status	Published - 2010

Bibliographical note

Funding Information:
This research was supported by the National Institutes of Health (P41-RR02594-18). Park was supported by a Samsung Scholarship. Diez-Silva and Suresh acknowledge support from the Interdisciplinary Research Group on Infectious Diseases, which is supported by the Singapore-MIT Alliance for Research and Technology and by the National Institutes of Health (1R01HL094270-01A1).

Keywords

Electric field
Light scattering
Malaria
Plasmodium falciparum
Red blood cell

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.3369966

Cite this

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title = "Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells",

abstract = "We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.",

keywords = "Electric field, Light scattering, Malaria, Plasmodium falciparum, Red blood cell",

author = "Park, {Yong Keun} and Monica Diez-Silva and Dan Fu and Gabriel Popescu and Wonshik Choi and Ishan Barman and Subra Suresh and Feld, {Michael S.}",

note = "Funding Information: This research was supported by the National Institutes of Health (P41-RR02594-18). Park was supported by a Samsung Scholarship. Diez-Silva and Suresh acknowledge support from the Interdisciplinary Research Group on Infectious Diseases, which is supported by the Singapore-MIT Alliance for Research and Technology and by the National Institutes of Health (1R01HL094270-01A1).",

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AU - Park, Yong Keun

AU - Diez-Silva, Monica

AU - Fu, Dan

AU - Popescu, Gabriel

AU - Choi, Wonshik

AU - Barman, Ishan

AU - Suresh, Subra

AU - Feld, Michael S.

N1 - Funding Information: This research was supported by the National Institutes of Health (P41-RR02594-18). Park was supported by a Samsung Scholarship. Diez-Silva and Suresh acknowledge support from the Interdisciplinary Research Group on Infectious Diseases, which is supported by the Singapore-MIT Alliance for Research and Technology and by the National Institutes of Health (1R01HL094270-01A1).

PY - 2010

Y1 - 2010

N2 - We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.

AB - We present the light scattering of individual Plasmodium falciparum-parasitized human red blood cells (Pf-RBCs), and demonstrate progressive alterations to the scattering signal arising from the development of malaria-inducing parasites. By selectively imaging the electric fields using quantitative phase microscopy and a Fourier transform light scattering technique, we calculate the light scattering maps of individual Pf-RBCs. We show that the onset and progression of pathological states of the Pf-RBCs can be clearly identified by the static scattering maps. Progressive changes to the biophysical properties of the Pf-RBC membrane are captured from dynamic light scattering.

KW - Electric field

KW - Light scattering

KW - Malaria

KW - Plasmodium falciparum

KW - Red blood cell

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Static and dynamic light scattering of healthy and malaria-parasite invaded red blood cells

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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