Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

J. Y. Min; M. H. Park; M. K. Park; K. W. Park; N. W. Lee; T. Kim; H. J. Kim; D. H. Lee

doi:10.1007/s00702-006-0500-z

Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

J. Y. Min
, M. H. Park^*
, M. K. Park
, K. W. Park
, N. W. Lee
, T. Kim
, H. J. Kim
, D. H. Lee

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H₂O₂ causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms

Original language	English
Pages (from-to)	1821-1826
Number of pages	6
Journal	Journal of Neural Transmission
Volume	113
Issue number	11
DOIs	https://doi.org/10.1007/s00702-006-0500-z
Publication status	Published - 2006 Nov
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Neurite outgrowth
PKC
ROS
Staurosporin

ASJC Scopus subject areas

Neurology
Clinical Neurology
Psychiatry and Mental health
Biological Psychiatry

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10.1007/s00702-006-0500-z

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title = "Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines",

abstract = "Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms",

keywords = "Neurite outgrowth, PKC, ROS, Staurosporin",

author = "Min, \{J. Y.\} and Park, \{M. H.\} and Park, \{M. K.\} and Park, \{K. W.\} and Lee, \{N. W.\} and T. Kim and Kim, \{H. J.\} and Lee, \{D. H.\}",

year = "2006",

month = nov,

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language = "English",

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TY - JOUR

T1 - Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

AU - Min, J. Y.

AU - Park, M. H.

AU - Park, M. K.

AU - Park, K. W.

AU - Lee, N. W.

AU - Kim, T.

AU - Kim, H. J.

AU - Lee, D. H.

PY - 2006/11

Y1 - 2006/11

N2 - Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms

AB - Staurosporin, a specific inhibitor of PKC, is widely used in studies of signal transduction pathways. Previous studies have shown that staurosporin induces neurite outgrowth, but the underlying mechanisms remain unclear. Here we report that staurosporin induces neurite outgrowth in HN33 hippocampal cells. Two other PKC inhibitors, Go 6976 (specific for α- and β-isoforms) and rotterlin (a selective inhibitor of PKC δ), have no neuritogenic effect. In addition, staurosporin specifically increases ROS generation. NAC, which inhibits the generation of ROS, suppresses the staurosporin-induced neurite outgrowth in HN33 cells. Further, H2O2 causes neurite outgrowth. Taken together, these results confirm a neuritogenic effect of staurosporin and point to ROS as the signal mediator of staurosporin-induced neurite outgrowth in HN33 hippocampal cells. Theme: Development and regeneration Topic: Neurotrophic factors: receptors and cellular mechanisms

KW - Neurite outgrowth

KW - PKC

KW - ROS

KW - Staurosporin

UR - https://www.scopus.com/pages/publications/33750453367

U2 - 10.1007/s00702-006-0500-z

DO - 10.1007/s00702-006-0500-z

M3 - Article

C2 - 16715208

AN - SCOPUS:33750453367

SN - 0300-9564

VL - 113

SP - 1821

EP - 1826

JO - Journal of Neural Transmission

JF - Journal of Neural Transmission

IS - 11

ER -

Staurosporin induces neurite outgrowth through ROS generation in HN33 hippocampal cell lines

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

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