Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Sang Jun Lee; Laxmi Prasad Thapa; Ju Hun Lee; Han Suk Choi; Sung Bong Kim; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.procbio.2016.09.008

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Sang Jun Lee, Laxmi Prasad Thapa, Ju Hun Lee, Han Suk Choi, Sung Bong Kim, Chulhwan Park, Seung Wook Kim

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

Original language	English
Pages (from-to)	1904-1910
Number of pages	7
Journal	Process Biochemistry
Volume	51
Issue number	12
DOIs	https://doi.org/10.1016/j.procbio.2016.09.008
Publication status	Published - 2016 Dec 1

Keywords

2,3-Butanediol
Acetate
Enterobacter aerogenes
Fermentation
Transcriptional regulation

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2016.09.008

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title = "Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007",

abstract = "2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.",

keywords = "2,3-Butanediol, Acetate, Enterobacter aerogenes, Fermentation, Transcriptional regulation",

author = "Lee, {Sang Jun} and Thapa, {Laxmi Prasad} and Lee, {Ju Hun} and Choi, {Han Suk} and Kim, {Sung Bong} and Chulhwan Park and Kim, {Seung Wook}",

note = "Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of Global Frontier Project funded by the Ministry of Science , ICT and Future Planning of Korea. This research was also supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2057887 ). Publisher Copyright: {\textcopyright} 2016 Elsevier Ltd",

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doi = "10.1016/j.procbio.2016.09.008",

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T1 - Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

AU - Lee, Sang Jun

AU - Thapa, Laxmi Prasad

AU - Lee, Ju Hun

AU - Choi, Han Suk

AU - Kim, Sung Bong

AU - Park, Chulhwan

AU - Kim, Seung Wook

N1 - Funding Information: This work was supported by the Advanced Biomass R&D Center (ABC-2011-0031360) of Global Frontier Project funded by the Ministry of Science , ICT and Future Planning of Korea. This research was also supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education ( NRF-2013R1A1A2057887 ). Publisher Copyright: © 2016 Elsevier Ltd

PY - 2016/12/1

Y1 - 2016/12/1

N2 - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

AB - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

KW - 2,3-Butanediol

KW - Acetate

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KW - Fermentation

KW - Transcriptional regulation

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VL - 51

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JO - Process Biochemistry

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ER -

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Abstract

Keywords

ASJC Scopus subject areas

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