Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Sang Jun Lee; Laxmi Prasad Thapa; Ju Hun Lee; Han Suk Choi; Sung Bong Kim; Chulhwan Park; Seung Wook Kim

doi:10.1016/j.procbio.2016.09.008

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Sang Jun Lee
, Laxmi Prasad Thapa
, Ju Hun Lee
, Han Suk Choi
, Sung Bong Kim
, Chulhwan Park^*
, Seung Wook Kim

^*Corresponding author for this work

Department of Chemical and Biological Engineering

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

Original language	English
Pages (from-to)	1904-1910
Number of pages	7
Journal	Process Biochemistry
Volume	51
Issue number	12
DOIs	https://doi.org/10.1016/j.procbio.2016.09.008
Publication status	Published - 2016 Dec 1

Bibliographical note

Publisher Copyright:
© 2016 Elsevier Ltd

Keywords

2,3-Butanediol
Acetate
Enterobacter aerogenes
Fermentation
Transcriptional regulation

ASJC Scopus subject areas

Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.procbio.2016.09.008

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title = "Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007",

abstract = "2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2\% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.",

keywords = "2,3-Butanediol, Acetate, Enterobacter aerogenes, Fermentation, Transcriptional regulation",

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doi = "10.1016/j.procbio.2016.09.008",

language = "English",

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T1 - Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

AU - Lee, Sang Jun

AU - Thapa, Laxmi Prasad

AU - Lee, Ju Hun

AU - Choi, Han Suk

AU - Kim, Sung Bong

AU - Park, Chulhwan

AU - Kim, Seung Wook

PY - 2016/12/1

Y1 - 2016/12/1

N2 - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

AB - 2,3-Butanediol is an attractive product for biorefinery due to its various applications and industrial potential. Enterobacter aerogenes is also considered as a major 2,3-butanediol producer. This study is intended to enhance the production of 2,3-butanediol with addition of acetate into the culture media using E. aerogenes ATCC 29007 and to determine the mechanism of improved 2,3-butanediol production. 2,3-Butanediol production was increased by 34.2% with the addition of acetate in batch culture compared to that without acetate in 130 g/L of glucose. This increase was caused by buffering effects and upregulation of the transcription levels of genes involved in 2,3-butanediol production through acetate addition.

KW - 2,3-Butanediol

KW - Acetate

KW - Enterobacter aerogenes

KW - Fermentation

KW - Transcriptional regulation

UR - https://www.scopus.com/pages/publications/84994504366

U2 - 10.1016/j.procbio.2016.09.008

DO - 10.1016/j.procbio.2016.09.008

M3 - Article

AN - SCOPUS:84994504366

SN - 1359-5113

VL - 51

SP - 1904

EP - 1910

JO - Process Biochemistry

JF - Process Biochemistry

IS - 12

ER -

Stimulation of 2,3-butanediol production by upregulation of alsR gene transcription level with acetate addition in Enterobacter aerogenes ATCC 29007

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

Access to Document

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