Strain development and medium optimization for fumaric acid production

Seong Woo Kang, Hawon Lee, Daeheum Kim, Dohoon Lee, Sangyong Kim, Gie Taek Chun, Jinwon Lee, Seung Wook Kim, Chulhwan Park

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Rhizopus oryzae RUR709 mutant was isolated based on halo size from selection medium via mutagenesis with UV and γ-rays, and the production of fumaric acid in the submerged fermentation was assessed. The maximum concentration of fumaric acid was obtained using 0.5% corn steep liquor (CSL) as the nitrogen source. Organic nitrogen sources were shown to be more effective in fumaric acid production than inorganic nitrogen sources. Using optimum medium obtained by response surface methodology (RSM), the maximum concentration of fumaric acid achieved in flask culture was 26.2 g/L, which is fairly close to the 27.4 g/L predicted by the model. The highest concentration of fumaric acid in the stirred-tank reactor generated by the R. oryzae RUR709 mutant was 32.1 g/L and yield (0.45 g/g) and productivity (0.32 g/L/h) were highest at 4 days.

Original languageEnglish
Pages (from-to)761-769
Number of pages9
JournalBiotechnology and Bioprocess Engineering
Issue number5
Publication statusPublished - 2010 Oct

Bibliographical note

Funding Information:
The authors gratefully acknowledge the financial support provided by the Korea Energy Management Cooperation (KEMCO). This research was also supported by a Research Grant from Kwangwoon University in 2008.


  • Rhizopus oryzae
  • fumaric acid
  • mutagenesis
  • optimization
  • response surface methodology (RSM)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering


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