Strategy for high-yield astaxanthin recovery directly from wet Haematococcus pluvialis without pretreatment

Aye Aye Myint, Patrick Hariyanto, Muhammad Irshad, Cao Ruqian, Sabrinna Wulandari, Min Eui Hong, Sang Jun Sim, Jaehoon Kim

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


A novel integrated extraction technique for high recovery of natural astaxanthin from wet encysted Haematococcus pluvialis (H. pluvialis) is demonstrated. The technique can be used to effectively disrupt the cell wall and perform extraction in a one-pot system without a high-energy, cost intensive pre-drying step. The most suitable green solvent was researched in terms of high extraction yield and astaxanthin recovery. Moreover, an optimized condition for the selected green solvents was determined by varying process parameters, viz., the ball milling speed (100–300 rpm) and time (5–30 min). A high recovery of astaxanthin directly from wet H. pluvialis (30.6 mg/g based on its dry mass) and a high extraction yield (58.2 wt%) were achieved using ethyl acetate at 200 rpm after 30 min. Therefore, compared to its counterparts, the biphasic solvent system plays a key role in achieving high extraction yield and astaxanthin recovery from wet H. pluvialis.

Original languageEnglish
Article number126616
JournalBioresource technology
Publication statusPublished - 2022 Feb

Bibliographical note

Funding Information:
This work was supported by the Energy Efficiency & Resources Core Technology Program of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) and the granted financial resource from the Ministry of Trade, Industry & Energy, Republic of Korea (Project No.: 202020202800330). The authors also acknowledge a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (Project No.: 2018R1D1A1B07047129) and Brain Pool program (Project No.: 2020H1D3A2A02043653) for additional support to this work.

Publisher Copyright:
© 2021 Elsevier Ltd


  • Antioxidant activities
  • Astaxanthin
  • Haematococcus pluvialis
  • Wet ball mill extraction

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal


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