Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.
|Number of pages||10|
|Journal||Journal of Biological Chemistry|
|Publication status||Published - 2013 Aug 9|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology