Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei

Kwang Hoon Sung, So Yeon Lee, Hyun Kyu Song

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    7 Citations (Scopus)

    Abstract

    Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.

    Original languageEnglish
    Pages (from-to)23234-23243
    Number of pages10
    JournalJournal of Biological Chemistry
    Volume288
    Issue number32
    DOIs
    Publication statusPublished - 2013 Aug 9

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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