Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei

Kwang Hoon Sung; So Yeon Lee; Hyun Kyu Song

doi:10.1074/jbc.M113.484832

Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei

Kwang Hoon Sung, So Yeon Lee, Hyun Kyu Song

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.

Original language	English
Pages (from-to)	23234-23243
Number of pages	10
Journal	Journal of Biological Chemistry
Volume	288
Issue number	32
DOIs	https://doi.org/10.1074/jbc.M113.484832
Publication status	Published - 2013 Aug 9

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1074/jbc.M113.484832

Cite this

@article{238582e24790429fbeee72b55887b21b,

title = "Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei",

abstract = "Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 {\AA} resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.",

author = "Sung, {Kwang Hoon} and Lee, {So Yeon} and Song, {Hyun Kyu}",

year = "2013",

month = aug,

day = "9",

doi = "10.1074/jbc.M113.484832",

language = "English",

volume = "288",

pages = "23234--23243",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology Inc.",

number = "32",

}

TY - JOUR

T1 - Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei

AU - Sung, Kwang Hoon

AU - Lee, So Yeon

AU - Song, Hyun Kyu

PY - 2013/8/9

Y1 - 2013/8/9

N2 - Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.

AB - Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.

UR - http://www.scopus.com/inward/record.url?scp=84881398584&partnerID=8YFLogxK

U2 - 10.1074/jbc.M113.484832

DO - 10.1074/jbc.M113.484832

M3 - Article

C2 - 23818520

AN - SCOPUS:84881398584

SN - 0021-9258

VL - 288

SP - 23234

EP - 23243

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 32

ER -

Structural and biochemical analyses of the eukaryotic heat shock locus v (HslV) from Trypanosoma brucei

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this