Abstract
Background: A eukaryotic HslV (TbHslV) protease and two potential HslU (TbHslU1 and TbHslU2) ATPases have been isolated from Trypanosoma brucei. Results: We determined the crystal structure of TbHslV at 2.4 Å resolution. Only TbHslU2 activated TbHslV protease activity. Conclusion: A key tyrosine residue in TbHslU2 required for activating TbHslV was identified. Significance: This study lays the groundwork for understanding the eukaryotic HslVU system.
Original language | English |
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Pages (from-to) | 23234-23243 |
Number of pages | 10 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 32 |
DOIs | |
Publication status | Published - 2013 Aug 9 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology