Structural and Functional Insights into Dom34, a Key Component of No-Go mRNA Decay

Hyung Ho Lee, Youn Sung Kim, Kyoung Hoon Kim, Inha Heo, Sang Kyu Kim, Olesya Kim, Hye Kyung Kim, Ji Young Yoon, Hyoun Sook Kim, Do Jin Kim, Sang Jae Lee, Hye Jin Yoon, Soon Jong Kim, Byung Gil Lee, Hyun Kyu Song, V. Narry Kim, Chung Mo Park, Se Won Suh

Research output: Contribution to journalArticlepeer-review

78 Citations (Scopus)


The yeast protein Dom34 is a key component of no-go decay, by which mRNAs with translational stalls are endonucleolytically cleaved and subsequently degraded. However, the identity of the endoribonuclease is unknown. Homologs of Dom34, called Pelota, are broadly conserved in eukaryotes and archaea. To gain insights into the structure and function of Dom34/Pelota, we have determined the structure of Pelota from Thermoplasma acidophilum (Ta Pelota) and investigated the ribonuclease activity of Dom34/Pelota. The structure of Ta Pelota is tripartite, and its domain 1 has the RNA-binding Sm fold. We have discovered that Ta Pelota has a ribonuclease activity and that its domain 1 is sufficient for the catalytic activity. We also demonstrate that domain 1 of Dom34 has an endoribonuclease activity against defined RNA substrates containing a stem loop, which supports a direct catalytic role of yeast Dom34 in no-go mRNA decay.

Original languageEnglish
Pages (from-to)938-950
Number of pages13
JournalMolecular Cell
Issue number6
Publication statusPublished - 2007 Sept 21


  • RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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