Structural and immunological feature of rhamnogalacturonan I-rich polysaccharide from Korean persimmon vinegar

Hoon Kim, Hee Do Hong, Hyung Joo Suh, Kwang Soon Shin

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)


The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-α, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000 Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using β-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65–70%) derived from persimmon as a raw material, a mannan (20–25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar.

Original languageEnglish
Pages (from-to)319-327
Number of pages9
JournalInternational Journal of Biological Macromolecules
Publication statusPublished - 2016 Aug 1

Bibliographical note

Funding Information:
This study was supported by The Globalization of Korean Foods R&D Program of the Ministry of Agriculture and Food and Rural Affairs . Also, a part of this study was supported by a grant from the Korean Food Research Institute (2015). We express our sincere gratitude for their supports.

Publisher Copyright:
© 2016 Elsevier B.V.


  • Macrophage stimulation
  • Mannan
  • Persimmon vinegar
  • Polysaccharide
  • Rhamnogalacturonan I

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • General Energy


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