Structural and immunological feature of rhamnogalacturonan I-rich polysaccharide from Korean persimmon vinegar

Hoon Kim, Hee Do Hong, Hyung Joo Suh, Kwang Soon Shin

    Research output: Contribution to journalArticlepeer-review

    30 Citations (Scopus)

    Abstract

    The crude polysaccharide (KPV-0) isolated from Korean persimmon vinegar was fractionated using gel filtration chromatography to enhance the immunostimulatory activity and to identify the structural features of active fraction. Among three fractions, KPV-I obtained in a void volume, demonstrated the potent production of macrophage-stimulating mediators, including tumor necrosis factor-α, interleukin (IL)-6, IL-12, and nitric oxide. KPV-I showed a combined single peak with high molecular weight of 55,000 Da by high performance size exclusion chromatography. Component sugar analysis revealed that KPV-I contained mainly of arabinose, mannose, galactose, rhamnose and galacturonic acid. Single radial gel diffusion assay using β-glucosyl Yariv reagent showed that KPV-I contained arabinogalactan protein with 13.7%. Methylation analysis indicated that KPV-I contained 21 kinds of neutral glycosidic linkages, which seemed to be composed three kinds of polysaccharide; that is a rhamnogalacturonan-I (65–70%) derived from persimmon as a raw material, a mannan (20–25%) derived from fermentation-associated microorganisms, and a linear glucans (less than 10%). In conclusion, polysaccharide isolated from persimmon vinegar could augment the macrophage stimulation, and a large amounts of RG-I polysaccharide derived from persimmon is likely a crucial role in expression of the activity in persimmon vinegar.

    Original languageEnglish
    Pages (from-to)319-327
    Number of pages9
    JournalInternational Journal of Biological Macromolecules
    Volume89
    DOIs
    Publication statusPublished - 2016 Aug 1

    Bibliographical note

    Funding Information:
    This study was supported by The Globalization of Korean Foods R&D Program of the Ministry of Agriculture and Food and Rural Affairs . Also, a part of this study was supported by a grant from the Korean Food Research Institute (2015). We express our sincere gratitude for their supports.

    Publisher Copyright:
    © 2016 Elsevier B.V.

    Keywords

    • Macrophage stimulation
    • Mannan
    • Persimmon vinegar
    • Polysaccharide
    • Rhamnogalacturonan I

    ASJC Scopus subject areas

    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Economics and Econometrics
    • General Energy

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