Structure-based identification of a novel NTPase from Methanococcus jannaschii

Kwang Yeon Hwang, Ji Hyung Chung, Sung Hou Kim, Ye Sun Han, Yunje Cho

Research output: Contribution to journalArticlepeer-review

124 Citations (Scopus)


Almost half of the entire set of predicted genomic products from Methanococcus jannaschii are classified as functionally unknown hypothetical proteins. We present a structure-based identification of the biochemical function of a protein with an as yet unknown function from a M. jannaschii gene, Mj0226. The crystal structure of Mj0226 protein determined at 2.2 Å resolution reveals that the protein is a homodimer and each monomer folds into an elongated α/β structure of a new fold family. Comparisons of Mj0226 protein with protein structures in the database, however, indicate that one part of the protein is homologous to some of the nucleotide-binding proteins. Biochemical analysis shows that Mj0226 protein is a novel nucleotide triphosphatase that can efficiently hydrolyze nonstandard nucleotides such as XTP to XMP or ITP to IMP, but not the standard nucleotides, in the presence of Mg2+ or Mn2+ ions.

Original languageEnglish
Pages (from-to)691-696
Number of pages6
JournalNature Structural Biology
Issue number7
Publication statusPublished - 1999

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics


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