Abstract
We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.
Original language | English |
---|---|
Pages (from-to) | 4005-4008 |
Number of pages | 4 |
Journal | Antimicrobial Agents and Chemotherapy |
Volume | 56 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2012 Jul |
ASJC Scopus subject areas
- Pharmacology
- Pharmacology (medical)
- Infectious Diseases