Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del

Hyojeong Yi, Karan Kim, Kwang Hwi Cho, Oksung Jung, Heenam Stanley Kim

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

    Original languageEnglish
    Pages (from-to)4005-4008
    Number of pages4
    JournalAntimicrobial Agents and Chemotherapy
    Volume56
    Issue number7
    DOIs
    Publication statusPublished - 2012 Jul

    ASJC Scopus subject areas

    • Pharmacology
    • Pharmacology (medical)
    • Infectious Diseases

    Fingerprint

    Dive into the research topics of 'Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del'. Together they form a unique fingerprint.

    Cite this