Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del

Hyojeong Yi; Karan Kim; Kwang Hwi Cho; Oksung Jung; Heenam Stanley Kim

doi:10.1128/AAC.00598-12

Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del

Hyojeong Yi
, Karan Kim
, Kwang Hwi Cho
, Oksung Jung
, Heenam Stanley Kim^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

Original language	English
Pages (from-to)	4005-4008
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	56
Issue number	7
DOIs	https://doi.org/10.1128/AAC.00598-12
Publication status	Published - 2012 Jul

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

ASJC Scopus subject areas

Pharmacology
Pharmacology (medical)
Infectious Diseases

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10.1128/AAC.00598-12

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abstract = "We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.",

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AU - Yi, Hyojeong

AU - Kim, Karan

AU - Cho, Kwang Hwi

AU - Jung, Oksung

AU - Kim, Heenam Stanley

PY - 2012/7

Y1 - 2012/7

N2 - We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

AB - We describe a deletion mutation in a class A β-lactamase, PenA, of Burkholderia thailandensis that extended the substrate spectrum of the enzyme to include ceftazidime. Glu168del was located in a functional domain called the omega loop causing expansion of the space in the loop, which in turn increased flexibility at the active site. This deletion mutation represents a rare but significant alternative mechanical path to substrate spectrum extension in PenA besides more common substitution mutations.

UR - https://www.scopus.com/pages/publications/84862552432

U2 - 10.1128/AAC.00598-12

DO - 10.1128/AAC.00598-12

M3 - Article

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AN - SCOPUS:84862552432

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ER -

Substrate spectrum extension of PenA in Burkholderia thailandensis with a single amino acid deletion, Glu168del

Abstract

UN SDGs

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